Development of a Novel Membrane Aerated Hollow-Fiber Microbioreactor

详细信息    查看全文

• 作者：Louis Villain ; Lina Meyer ; Stephen Kroll ; Sascha Beutel ; Thomas Scheper
• 刊名：Biotechnology Progress
• 出版年：2008
• 出版时间：April 2008
• 年：2008
• 卷：24
• 期：2
• 页码：367 - 371
• 全文大小：198K
• 年卷期：v.24,no.2(April 2008)
• ISSN：1520-6033

文摘

A new challenge in biotechnological processes is the development of flexible bioprocessingplatforms, allowing strain selection, facilitating scale-up and integrating separation steps.Miniaturization of such a cultivation system allows parallel use and the saving of resources butmakes the supply of oxygen to the cells difficult. In this work we present a membrane aeratedhollow-fiber microbioreactor (HFMBR) which consists of an acrylic glass module equippedwith two different types of membrane fibers. Fibers of polyethersulfone and polyvinyldifluoridewere used for substrate and oxygen supply, respectively. Cultivation of E. coli as model organismand production of His-tagged GFP were carried out in the extracapillary space of the membraneaerated HFMBR and compared with cultivations in shaking flask which are commonly used forscreening experiments. The measurement of the oxygen transfer capacity and the onlinemonitoring of the dissolved oxygen during the cultivation were performed using a fiber opticoxygen sensor. Online measurement of the optical density was also integrated to the bioreactor.Due to efficient oxygen transfer, a better cell growth than in the shaking flask experiments wasachieved, while no negative influence on the GFP productivity was observed in the membraneaerated bioreactor. Thus the feasibility of a future integrated downstreaming could also bedemonstrated.