Experimental and Theoretical Investigations on the Adsorption of 2'-deoxyguanosine Oxidation Products at Oxidized Boron-Doped Diamond Electrodes

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• 作者：Elodie Fortin ; Eric Vieil ; Pascal Mailley ; Sabine Szunerits ; Thierry Livache
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：May 15, 2007
• 年：2007
• 卷：79
• 期：10
• 页码：3741 - 3746
• 全文大小：294K
• 年卷期：v.79,no.10(May 15, 2007)
• ISSN：1520-6882

文摘

Electrochemical oxidation of 2'-deoxyguanosine has beenperformed on boron-doped diamond (BDD) electrodes,resulting in a strong adsorption of the formed oxidizedproducts onto the BDD surface. The adsorption behaviorhas been investigated by studying the electrochemicalbehavior of a redox probe ([IrCl₆]^3-) using cyclic voltammetry. The most probable situations are the formationof (A) an insulating adsorbed film resulting in a partiallyblocked electrode behavior, (B) a porous film, or (C) anoverall conductive film. Different parameters such as thestandard rate constant, the charge-transfer coefficient, theelectrode/adsorbed products/solution interface resistance, and the formal potential of the redox couple weredetermined. Through comparison of theoretical current-potential curves obtained by analytical calculations withexperimental cyclic voltammograms, we found that theoxidized products of 2'-deoxyguanosine form a continuousconductive film on BDD.