Lateral Diffusion from Ligand Dissociation and Rebinding at Surfaces

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• 作者：Alena M. Lieto ; B. Christoffer Lagerholm ; and Nancy L. Thompson
• 刊名：Langmuir
• 出版年：2003
• 出版时间：March 4, 2003
• 年：2003
• 卷：19
• 期：5
• 页码：1782 - 1787
• 全文大小：117K
• 年卷期：v.19,no.5(March 4, 2003)
• ISSN：1520-5827

文摘

The lateral diffusion coefficient of a ligand which repeatedly dissociates and rebinds to sites on a planarsurface is described. An analytical expression showing the manner in which the diffusion coefficient dependson the propensity for rebinding after dissociation, as the molecule moves through the solution and acrossthe surface, is derived. The diffusion coefficient is time dependent and ranges from the surface diffusioncoefficient (at time zero when a tagged molecule is placed on the surface) to the solution diffusion coefficient(at time infinity). The time-dependent diffusion coefficient depends only on the intrinsic dissociation rateand a "rebinding parameter", which in turn depends on a group of constants including the intrinsic associationand dissociation rates, the density of unoccupied surface binding sites, and the diffusion coefficient insolution.