Flunixin Residues in Milk after Intravenous Treatment of Dairy Cattle with 14C-Flunixin
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- 作者：William F. Feely ; Cheryl Chester-Yansen ; Kenneth Thompson ; John W. Campbell ; Pamela L. Boner ; David D. W. Liu ; and Louis S. Crouch
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2002
- 出版时间：December 4, 2002
- 年：2002
- 卷：50
- 期：25
- 页码：7308 - 7313
- 全文大小：110K
- 年卷期：v.50,no.25(December 4, 2002)
- ISSN：1520-5118
文摘
Flunixin meglumine is used in veterinary medicine as an alternative to narcotic analgesics and as anantiinflammatory agent. Eight Holstein dairy cows were dosed intravenously once daily on threeconsecutive days with 14C-flunixin meglumine at approximately 2.2 mg of flunixin free acid/kg of bodyweight. Milk was collected twice daily to determine the decline of the total radioactive residues (TRR)in milk and to identify or characterize residue components. TRR in milk declined rapidly and averaged66, 20, and 14 ppb, respectively, for the first, second, and third milkings after administration of thelast dose. Milk was extracted, and the extracts were examined for radioactive residues. Meanextractability of milk TRR was always greater than 80%. Flunixin and 5-hydroxyflunixin were identifiedby coelution with analytical standards using reverse phase HPLC. These two residues were the mainradioactive residues found in milk and together accounted for 64, 37, and 44% of the extractableresidues, for the first, second, and third milkings, respectively, after administration of the last dose.The presence of 5-OH flunixin in milk was confirmed by HPLC/MS/MS.Keywords: Flunixin meglumine; 5-hydroxyflunixin; flunixin; HPLC/MS/MS; total radioactive residues