Effect of the Structure and Length of Flexible Chains on Dendrimers Grafted Fe3O4@SiO2/PAMAM Magnetic Nanocarriers for Lipase Immobilization

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• 作者：Panpan Zhao ; Lei Tian ; Xue Li ; Zafar Ali ; Baoliang Zhang ; Hepeng Zhang ; Qiuyu Zhang
• 刊名：ACS Sustainable Chemistry & Engineering
• 出版年：2016
• 出版时间：December 5, 2016
• 年：2016
• 卷：4
• 期：12
• 页码：6382-6390
• 全文大小：697K
• ISSN：2168-0485

文摘

Polyamidoamine (PAMAM) dendrimers have been widely applied in biomacromolecule immobilization, catalysis carriers, gene therapy, and drug delivery. However, there is still a lack of systematic research about lipase and protein immobilized by PAMAM dendrimers. Herein, we used three types of amine reagents to graft dendritic macromolecules on Fe₃O₄ nanoparticles and obtained a variety of Fe₃O₄@SiO₂/PAMAM magnetic nanocarriers with different generations. The density of surface functional groups, the structure, and the length (generation) of the flexible chain play an important role in immobilizing Candida rugosa lipase (CRL). As a result, hexamethylenediamine (HMD) grafted dendritic magnetic carriers with the fourth generation (Gc-4) exhibited a superior performance in terms of immobilizing CRL. Then, polyethylenimine (PEI) instead of HMD was grafted to the Gc-4 to obtain a higher activity with respect to immobilized CRL (955.53 U/g) and free lipase. Furthermore, the immobilized CRL improved its tolerability performance in wider ranges of pH and temperature.