Odd鈥揈ven Effect of Repeating Aminoethylene Units in the Side Chain of N-Substituted Polyaspartamides on Gene Transfection Profiles

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• 作者：Hirokuni Uchida ; Kanjiro Miyata ; Makoto Oba ; Takehiko Ishii ; Tomoya Suma ; Keiji Itaka ; Nobuhiro Nishiyama ; Kazunori Kataoka
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：October 5, 2011
• 年：2011
• 卷：133
• 期：39
• 页码：15524-15532
• 全文大小：1032K
• 年卷期：v.133,no.39(October 5, 2011)
• ISSN：1520-5126

文摘

A series of the N-substituted polyaspartamides possessing repeating aminoethylene units in the side chain was prepared in this study to identify polyplexes with effective endosomal escape and low cytotoxicity. All cationic N-substituted polyaspartamides showed appreciably lower cytotoxicity than that of commercial transfection reagents. Interestingly, a distinctive odd鈥揺ven effect of the repeating aminoethylene units in the polymer side chain on the efficiencies of endosomal escape and transfection to several cell lines was observed. The polyplexes from the polymers with an even number of repeating aminoethylene units (PA-Es) achieved an order of magnitude higher transfection efficiency, without marked cytotoxicity, than those of the polymers with an odd number of repeating aminoethylene units (PA-Os). This odd鈥揺ven effect agreed well with the buffering capacity of these polymers as well as their capability to disrupt membrane integrity selectively at endosomal pH, leading to highly effective endosomal escape of the PA-E polyplexes. Furthermore, the formation of a polyvalent charged array with precise spacing between protonated amino groups in the polymer side chain was shown to be essential for effective disruption of the endosomal membrane, thus facilitating transport of the polyplex into the cytoplasm. These data provide useful knowledge for designing polycations to construct safe and efficient nonviral gene carriers.