Surface-Scribed Transparency-Based Microplates

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• 作者：Xin Ye Li ; Brandon Huey-Ping Cheong ; Anthony Somers ; Oi Wah Liew ; Tuck Wah Ng
• 刊名：Langmuir
• 出版年：2013
• 出版时间：January 15, 2013
• 年：2013
• 卷：29
• 期：2
• 页码：849-855
• 全文大小：381K
• 年卷期：v.29,no.2(January 15, 2013)
• ISSN：1520-5827

文摘

Transparency sheets, which are normally associated with use on overhead projectors, offer lowered costs and high amenability for optical diagnostics in microplate instrumentation. An alternative microplate design in which circles are scribed on the surface of the transparency to create the boundaries to hold the drop in place is investigated here. The 3D profile of the scribed regions obtained optically showed strong likelihood of affecting three-phase contact line interactions. During dispensation, the contact angle (鈮?5掳) was larger than the drop advancing state (鈮?0掳) due to a period of nonadhesion, where the contact angle later reduced to the drop advancing state followed by increase in the liquid area coverage on the substrate. It was established that 50 渭L was needed to fill the well fully, and the maximum volume retainable before breaching was 190 渭L. While the tilt angle needed for displacement reduced significantly from 50 to 95 渭L, this was markedly better than nonscribed surfaces, where tilt angles always had to be kept to within 30掳. It was found that there was greater ability to fill the well with smaller volumes with dispensation at the center. This was attributed to the growing contact line not meeting the scribed edge in parallel if liquid was dispensed closer to it, wherein pinning reduction in some directions permitted liquid travel along the scribed edge to undergo contact angle hysteresis. Fluorescence measurements conducted showed no performance compromise when using scribed transparency microplates over standard microplates.