Synthesis of a Fluorescent Xanthenic Derivative Useful for Labeling Amine Residues

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• 作者：Luis Crovetto ; Ramon Rios ; Jose M. Alvarez-Pez ; Jose M. Paredes ; Patricia Lozano-Velez ; Carmen del Valle ; Eva M. Talavera
• 刊名：Journal of Physical Chemistry B
• 出版年：2008
• 出版时间：August 21, 2008
• 年：2008
• 卷：112
• 期：33
• 页码：10082-10085
• 全文大小：117K
• 年卷期：v.112,no.33(August 21, 2008)
• ISSN：1520-5207

文摘

The 2,5-dioxopyrrolidin-1-yl-4-(3-hydroxy-6-oxo-6H-xanthen-9-yl)-3-methylbenzoate has been synthesized as an amine-reactive derivative able to yield stable covalently labeled biopolymers. The new derivative has been used to label polyribocytidilic acid (5′), poly(C), amine residues. TG-II-poly(C) exhibits monoexponential decay at the physiological pH range. In addition, both steady-state fluorescence intensity and fluorescence decay are also sensitive to solution pH. The large decrease in steady-state fluorescence upon hybridization allows it to be used as a nucleic acid probe in a homogeneous assay format. In summary, we report an efficient synthesis to obtain labeled RNA from commercially available materials in excellent yields.