Induced Resistance in Tomato Plants against Verticillium Wilt by the Binuclear Nickel Coordination Complex of the Ligand 2,5-Bis(pyridin-2-yl)-1,3,4-thiadiazole

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• 作者：Hanane Zine ; Lalla Aicha Rifai ; Mohamed Faize ; Fouad Bentiss ; Salaheddine Guesmi ; Abdelhakim Laachir ; Amal Smaili ; Kacem Makroum ; Abdelaziz Sahibed-Dine ; Tayeb Koussa
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：April 6, 2016
• 年：2016
• 卷：64
• 期：13
• 页码：2661-2667
• 全文大小：389K
• ISSN：1520-5118

文摘

Verticillium wilt caused by Verticillium dahliae is a major limiting factor for tomato production. The objective of this study was to evaluate the effectiveness of ligand 2,5-bis(pyridin-2-yl)-1,3,4-thiadiazole (L) and its complex bis[μ-2,5-bis(pyridin-2-yl)-1,3,4-thiadiazole-κ⁴N²,N³:N⁴,N⁵]bis[dihydrato-κO)nickel(II)] as activators of plant defenses in controlling Verticillium wilt. In the greenhouse, they protected tomato plants against V. dahliae when they were applied twice as foliar sprays at 100 μg mL^–1. A synergistic effect was observed between the ligand L and the transition metal Ni, with disease incidence reduced by 38% with L and 57% with Ni₂L₂. Verticillium wilt foliar symptoms and vascular browning index were reduced by 82% for L and 95% for Ni₂L₂. This protection ability was associated with the induction of an oxidative burst and the activation of the total phenolic content as well as potentiation of the activity of peroxidase and polyphenol oxidase. These results demonstrated that L and Ni₂L₂ can be considered as new activators of plant defense responses.