文摘
Polypeptides are successfully incorporated into poly(pan class="smallcaps">lpan>-lactide) (PLLA) chains in a ring-opening polymerization (ROP) of pan class="smallcaps">lpan>-lactide by using them as initiators. The resulting ABA triblock copolymers possess molecular weights up to 11000 g路molp>鈥?p> and polydispersities as low as 1.13, indicating the living character of the polymerization process. In a nonaqueous emulsion, peptide-initiated polymerization of pan class="smallcaps">lpan>-lactide leads to well-defined nanoparticles, consisting of PLLA-block-peptide-block-PLLA copolymer. These nanoparticles are easily loaded by dye-encapsulation and transferred into aqueous media without aggregation (average diameter of 100 nm) or significant dye leakage. Finally, internalization of PLLA-block-peptide-block-PLLA nanoparticles by HeLa cells is demonstrated by a combination of coherent anti-Stokes Raman spectroscopy (CARS) and fluorescence microscopy. This demonstrates the promise of their utilization as cargo delivery vehicles.