Detection of Genistein as an Estrogenic Contaminant of River Water in Osaka
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- 作者：Masanobu Kawanishi ; Takeji Takamura-Enya ; Rahyani Ermawati ; Chiaki Shimohara ; Michiyo Sakamoto ; Kazuki Matsukawa ; Tomonari Matsuda ; Tsuyoshi Murahashi ; Saburo Matsui ; Keiji Wakabayashi ; Tetsushi Watan
- 刊名：Environmental Science & Technology
- 出版年：2004
- 出版时间：December 1, 2004
- 年：2004
- 卷：38
- 期：23
- 页码：6424 - 6429
- 全文大小：416K
- 年卷期：v.38,no.23(December 1, 2004)
- ISSN：1520-5851
文摘
The estrogenic activity in water at various localities onLake Biwa-Yodo River, a representative watershed inJapan, was measured using a recombinant yeast thatexpresses the human estrogen receptor. The yeast bioassayrevealed that the activities of 13 water samples had anaverage value of 14 pmol/L (3.8 ng/L) (17
-estradiol equivalent)with a very wide range from 0 to 72 pmol/L (0-19.6 ng/L), and two of the samples had prominent levels of activity(72 pmol/L (19.6 ng/L) and 56 pmol/L (15.2 ng/L)). Weanalyzed these two samples with instrumental approaches.A high-performance liquid chromatogram profile showedthat the strong activity in one sample, which was collectedjust downstream of a sewage-treatment plant, would bedue to 17-estradiol and estrone, whose source is consideredto be human urine contained in the effluent of the plant.The activity in the other sample, which was obtained froma tributary river in a primarily residential area with someindustrial development (i.e., Osaka City), however, did notcorrespond to 17-estradiol, estrone, or syntheticchemicals known as estrogenic. Analysis of a fractionwith estrogenic activity by liquid chromatography-massspectrometry (LC-MS) provided evidence that the activityin the water sample resulted from the presence ofgenistein, an isoflavone compound of plant origin.
-estradiol equivalent)with a very wide range from 0 to 72 pmol/L (0-19.6 ng/L), and two of the samples had prominent levels of activity(72 pmol/L (19.6 ng/L) and 56 pmol/L (15.2 ng/L)). Weanalyzed these two samples with instrumental approaches.A high-performance liquid chromatogram profile showedthat the strong activity in one sample, which was collectedjust downstream of a sewage-treatment plant, would bedue to 17-estradiol and estrone, whose source is consideredto be human urine contained in the effluent of the plant.The activity in the other sample, which was obtained froma tributary river in a primarily residential area with someindustrial development (i.e., Osaka City), however, did notcorrespond to 17-estradiol, estrone, or syntheticchemicals known as estrogenic. Analysis of a fractionwith estrogenic activity by liquid chromatography-massspectrometry (LC-MS) provided evidence that the activityin the water sample resulted from the presence ofgenistein, an isoflavone compound of plant origin.