Super-Resolution Fingerprinting Detects Chemical Reactions and Idiosyncrasies of Single DNA Pegboards
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- 作者：Alexander Johnson-Buck ; Jeanette Nangreave ; Do-Nyun Kim ; Mark Bathe ; Hao Yan ; Nils G. Walter
- 刊名：Nano Letters
- 出版年：2013
- 出版时间：February 13, 2013
- 年：2013
- 卷：13
- 期：2
- 页码：728-733
- 全文大小：413K
- 年卷期：v.13,no.2(February 13, 2013)
- ISSN：1530-6992
文摘
We employ the single-particle fluorescence nanoscopy technique points accumulation for imaging in nanoscale topography (PAINT) using site-specific DNA probes to acquire two-dimensional density maps of specific features patterned on nanoscale DNA origami pegboards. We show that PAINT has a localization accuracy of 10 nm that is sufficient to reliably distinguish dense (>104 features 渭m鈥?) sub-100 nm patterns of oligonucleotide features. We employ two-color PAINT to follow enzyme-catalyzed modification of features on individual origami and to show that single nanopegboards exhibit stable, spatially heterogeneous probe-binding patterns, or 鈥渇ingerprints.鈥?Finally, we present experimental and modeling evidence suggesting that these fingerprints may arise from feature spacing variations that locally modulate the probe binding kinetics. Our study highlights the power of fluorescence nanoscopy to perform quality control on individual soft nanodevices that interact with and position reagents in solution.