Trace-Level Mercury Ion (Hg2+) Analysis in Aqueous Sample Based on Solid-Phase Extraction Followed by Microfluidic Immunoassay

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• 作者：Yasumoto Date ; Arata Aota ; Shingo Terakado ; Kazuhiro Sasaki ; Norio Matsumoto ; Yoshitomo Watanabe ; Tomokazu Matsue ; Naoya Ohmura
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：January 2, 2013
• 年：2013
• 卷：85
• 期：1
• 页码：434-440
• 全文大小：336K
• 年卷期：v.85,no.1(January 2, 2013)
• ISSN：1520-6882

文摘

Mercury is considered the most important heavy-metal pollutant, because of the likelihood of bioaccumulation and toxicity. Monitoring widespread ionic mercury (Hg²⁺) contamination requires high-throughput and cost-effective methods to screen large numbers of environmental samples. In this study, we developed a simple and sensitive analysis for Hg²⁺ in environmental aqueous samples by combining a microfluidic immunoassay and solid-phase extraction (SPE). Using a microfluidic platform, an ultrasensitive Hg²⁺ immunoassay, which yields results within only 10 min and with a lower detection limit (LOD) of 0.13 渭g/L, was developed. To allow application of the developed immunoassay to actual environmental aqueous samples, we developed an ion-exchange resin (IER)-based SPE for selective Hg²⁺ extraction from an ion mixture. When using optimized SPE conditions, followed by the microfluidic immunoassay, the LOD of the assay was 0.83 渭g/L, which satisfied the guideline values for drinking water suggested by the United States Environmental Protection Agency (USEPA) (2 渭g/L; total mercury), and the World Health Organisation (WHO) (6 渭g/L; inorganic mercury). Actual water samples, including tap water, mineral water, and river water, which had been spiked with trace levels of Hg²⁺, were well-analyzed by SPE, followed by microfluidic Hg²⁺ immunoassay, and the results agreed with those obtained from reduction vaporizing鈥揳tomic adsorption spectroscopy.