Optochemogenetics (OCG) Allows More Precise Control of Genetic Engineering in Mice with CreER regulators

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• 作者：Xin Lu ; Sarit S. Agasti ; Claudio Vinegoni ; Peter Waterman ; Ronald A. DePinho ; Ralph Weissleder
• 刊名：Bioconjugate Chemistry
• 出版年：2012
• 出版时间：September 19, 2012
• 年：2012
• 卷：23
• 期：9
• 页码：1945-1951
• 全文大小：362K
• 年卷期：v.23,no.9(September 19, 2012)
• ISSN：1520-4812

文摘

New approaches that allow precise spatiotemporal control of gene expression in model organisms at the single cell level are necessary to better dissect the role of specific genes and cell populations in development, disease, and therapy. Here, we describe a new optochemogenetic switch (OCG switch) to control CreER/loxP-mediated recombination via photoactivatable (鈥砪aged鈥? tamoxifen analogues in individual cells in cell culture, organoid culture, and in vivo in adult mice. This approach opens opportunities to more fully exploit existing CreER transgenic mouse strains to achieve more precise temporal- and location-specific regulation of genetic events and gene expression.