A Visual Sensor Array for Pattern Recognition Analysis of Proteins Using Novel Blue-Emitting Fluorescent Gold Nanoclusters

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• 作者：Shenghao Xu ; Xin Lu ; Chenxi Yao ; Fu Huang ; Hua Jiang ; Wenhao Hua ; Na Na ; Haiyan Liu ; Jin Ouyang
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：December 2, 2014
• 年：2014
• 卷：86
• 期：23
• 页码：11634-11639
• 全文大小：370K
• ISSN：1520-6882

文摘

This paper describes a visual sensor array for pattern recognition analysis of proteins based on two different optical signal changes: colorimetric and fluorometric, by using two types of novel blue-emitting collagen protected gold nanoclusters and macerozyme R-10 protected gold nanoclusters with lower synthetic demands. Eight proteins have been well-discriminated by this visual sensor array, and protein mixtures after one-dimensional polyacrylamide gel electrophoresis also could be well-discriminated. The possible mechanism of this sensor array was illustrated and validated by fluorescence spectra, X-ray photoelectron spectroscopy (XPS), fluorescence lifetime, isothermal titration calorimetry (ITC), and matrix-assisted laser desorption/ionization鈥搕ime-of-flight mass spectrometry (MALDI-TOF MS) experiments. It was attributed to that the adsorption of proteins onto the surface of gold nanoclusters (Au NCs), forming the protein鈥揂u NCs complex. Furthermore, serums from normal and hepatoma patients were also effectively discriminated by this visual sensor array, showing feasible potential for diagnostic applications.