Biosynthesis of the Novel Macrolide Antibiotic Anthracimycin

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• 作者：Silke Alt ; Barrie Wilkinson
• 刊名：ACS Chemical Biology
• 出版年：2015
• 出版时间：November 20, 2015
• 年：2015
• 卷：10
• 期：11
• 页码：2468-2479
• 全文大小：610K
• ISSN：1554-8937

文摘

We report the identification of the biosynthetic gene cluster for the unusual antibiotic anthracimycin (atc) from the marine derived producer strain Streptomyces sp. T676 isolated off St. John鈥檚 Island, Singapore. The 53鈥?53 bps atc locus includes a trans-acyltransferase (trans-AT) polyketide synthase (PKS), and heterologous expression in Streptomyces coelicolor resulted in anthracimycin production. Analysis of the atc cluster revealed that anthracimycin is likely generated by four PKS gene products AtcC鈥揂tcF without involvement of post-PKS tailoring enzymes, and a biosynthetic pathway is proposed. The availability of the atc cluster provides a basis for investigating the biosynthesis of anthracimycin and its subsequent bioengineering to provide novel analogues with improved pharmacological properties.