Uniformly Aligned Full-Length Membrane Proteins in Liquid Crystalline Bilayers for Structural Characterization
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- 作者：Conggang Li ; Philip Gao ; Huajun Qin ; Rose Chase ; Peter L. Gor'kov ; William W. Brey ; Timothy A. Cross
- 刊名：Journal of the American Chemical Society
- 出版年：2007
- 出版时间：May 2, 2007
- 年：2007
- 卷：129
- 期：17
- 页码：5304 - 5305
- 全文大小：171K
- 年卷期：v.129,no.17(May 2, 2007)
- ISSN：1520-5126
文摘
High-resolution solid-state NMR spectra of three full-length membrane proteins uniformly aligned in lipid bilayers between glass slides are observed at high magnetic field. The resolution of the specific amino acid labeled samples shows promise for large membrane protein structure determination utilizing aligned samples and shows resonance patterns known as PISA wheels. The tilt angles of the transmembrane helices are extracted from the resonance patterns in PISEMA spectra.