Synthesis and Characterization of Dextran鈥揟yramine-Based H2O2-Sensitive Microgels

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• 作者：Hua Wei ; Jianda Xie ; Xiaomei Jiang ; Ting Ye ; Aiping Chang ; Weitai Wu
• 刊名：Macromolecules
• 出版年：2014
• 出版时间：September 9, 2014
• 年：2014
• 卷：47
• 期：17
• 页码：6067-6076
• 全文大小：523K
• ISSN：1520-5835

文摘

We report a type of polymer microgel that can undergo a rapid and highly sensitive volume change upon adding H₂O₂. Such a H₂O₂-sensitive microgel is made of dextran鈥搕yramine and horseradish peroxidase (HRP), which are interpenetrated in chemically cross-linked gel networks of poly(oligo(ethylene glycol) methacrylates). Unlike the H₂O₂-sensitive microgels reported in previous arts that typically involve degradation processes related to H₂O₂-induced cleavability of specific bonds, the proposed microgels can shrink upon adding H₂O₂ owing to the HRP-catalyzed coupling reaction of tyramine residues via decomposition of H₂O₂. While a fast (<10 s) and stable shrinkage of the microgels can be reached upon adding H₂O₂ over a concentration range 50.0 渭M鈥?.0 mM, the response time can be modulated by the dispersion temperature in a nonmonotonous way over 10鈥?8 掳C. With the microgels as probes, the H₂O₂ detection limit was approximately 6.8 渭M. In a combined use of the microgels with glucose oxidase for glucose detection, the glucose detection limit was approximately 83.1 渭M.