Synthesis of Oleoylethanolamide Using Lipase
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- 作者：Xiaosan Wang ; Xingguo Wang ; Tong Wang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：January 11, 2012
- 年：2012
- 卷：60
- 期：1
- 页码：451-457
- 全文大小：302K
- 年卷期：v.60,no.1(January 11, 2012)
- ISSN：1520-5118
文摘
An effective process for the enzymatic synthesis of oleoylethanolamide is described in this study. The process included purification of a commercial oleic acid product and then optimization of the reaction between the purified oleic acid and ethanolamine in the presence of hexane and a lipase. Under the optimal amidation reaction conditions identified, oleoylethanolamide was obtained with 96.6% purity. The synthesis was also conducted on a large scale (50 mmol of each of the reactants), and oleoylethanolamide purity and yield after crystallization purification were 96.1 and 73.5%, respectively. Compared to the previous studies, the current method of preparing high-purity oleoylethanolamide is more effective and economically feasible. The scalability and ease for such synthesis make it possible to study the biological and nutritional functions of the cannabinoid-like oleoylethanolamide in animal or human subjects.