Determination of Adulteration in Apple Juice by HPLC with Novel Optical Rotation Detector

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• 作者：Atsushi Yamamoto ; Mio Kawai ; Toshio Miwa ; Tomoyasu Tsukamoto ; Shuji Kodama ; Kazuichi Hayakawa
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：August 27, 2008
• 年：2008
• 卷：56
• 期：16
• 页码：7302-7304
• 全文大小：137K
• 年卷期：v.56,no.16(August 27, 2008)
• ISSN：1520-5118

文摘

A simple HPLC method for the determination of adulteration in apple juice was developed. The method is based on the detection of d-malate, derived from racemic malic acid, which is added as an acidulant. A variable-wavelength optical rotation detector was used to determine the enantiomeric excess (ee). Using anion-exchange chromatography with a phosphate buffer eluent and UV (210 nm) detection, the limit of detection for l-malate was 2 μg. With an injection of 13.4 μg of malate, the standard deviation of the ee calibration curve was 2.5%. Several apple juice samples were analyzed according to the proposed procedure, and the results agreed with those obtained using enzymatic kits for food analysis.