Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt

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• 作者：Bhavinkumar B. Patel ; Xin-Ming Li ; Maketa P. Dixon ; Elena L. Blagoi ; Steven H. Seeholzer ; Yibai Chen ; C. Glenn Miller ; Yin A. He ; Mazell Tetruashvily ; Anam H. Chaudhry ; Eileen Ke ; Joan Xie ; Harry Coop
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：June 2007
• 年：2007
• 卷：6
• 期：6
• 页码：2232 - 2238
• 全文大小：440K
• 年卷期：v.6,no.6(June 2007)
• ISSN：1535-3907

文摘

We seek alterations in protein patterns at the earliest possible step on the path to cancer, namely, incells of the target tissue from normal persons versus the corresponding normally appearing cells frompersons who are heterozygous for mutation in a tumor suppressor gene that predisposes strongly tocarcinoma in that tissue. To begin a systematic comparison of the proteomes of cells from normal andfrom neoplastic colons, we have undertaken the isolation of human colon crypts that are derived fromthe normal-appearing mucosa of left (descending) colon of patients with sporadic colorectal cancer.Two-dimensional (2D) gel electrophoresis is a proteomic approach that excels in the resolution of proteinisoforms. Here, we document the practicality of this approach with human samples using gels of threeoverlapping pH ranges. For the first time, about 800 nonredundant proteins and 900 isoforms frompurified human colonic crypts were identified, permitting an assessment of the contributions of proteinisoforms. These interactive, searchable, hyperlink-enabled proteome maps and gene ontology analyseswill facilitate future studies to discover the earliest markers and intervention targets during progressionto colon cancer.