文摘
A steam flash explosion (SFE) process followed by alkali extraction was applied for extracting keratin from duck feathers. Duck feathers were exploded at different steam pressures (1.4鈥?.0 MPa) for 0.5鈥? min. Results showed that combined SFE with alkaline treatment could effectively promote the dissolution of feather keratin. The optimal extraction conditions in this study were 0.4% NaOH, a solvent to material ratio of 20:1 (v/w), 25 掳C, and 1 h. An extraction rate of feathers of 65.78% and a yield of keratin of 42.78% was obtained when the steam exploded feather (1.6 MPa, 1 min) was treated under the above conditions. The extracted keratin retained most of the protein backbone, with the breakage of disulfide cross-links and hydrogen bonds. It was also found that the dissolution involved the fragmentation of macromolecular chains and a loss of some ordered structure. SFE assisted dissolution of keratin from feathers, without the use of harmful reagents, offers a possibility of large-scale exploitation.