Facile Coating Strategy to Functionalize Inorganic Nanoparticles for Biosensing

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• 作者：Yong Il Park ; Eunha Kim ; Chen-Han Huang ; Ki Soo Park ; Cesar M. Castro ; Hakho Lee ; Ralph Weissleder
• 刊名：Bioconjugate Chemistry
• 出版年：2017
• 出版时间：January 18, 2017
• 年：2017
• 卷：28
• 期：1
• 页码：33-37
• 全文大小：330K
• ISSN：1520-4812

文摘

The use of inorganic nanoparticles (NPs) for biosensing requires that they exhibit high colloidal stability under various physiological conditions. Here, we report on a general approach to render hydrophobic NPs into hydrophilic ones that are ready for bioconjugation. The method uses peglyated polymers conjugated with multiple dopamines, which results in multidentate coordination. As proof-of-concept, we applied the coating to stabilize ferrite and lanthanide NPs synthesized by thermal decomposition. Both polymer-coated NPs showed excellent water solubility and were stable at high salt concentrations under physiological conditions. We used these NPs as molecular-sensing agents to detect exosomes and bacterial nucleic acids.