Remote-Controlled Release of DNA in Living Cells via Simultaneous Light and Host鈥揋uest Mediations

详细信息    查看全文

• 作者：Jing Zheng ; Yuhong Nie ; Sheng Yang ; Yue Xiao ; Jishan Li ; Yinhui Li ; Ronghua Yang
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：October 21, 2014
• 年：2014
• 卷：86
• 期：20
• 页码：10208-10214
• 全文大小：370K
• ISSN：1520-6882

文摘

Using photons as external triggers to realize remote-controlled release of oligonucleotide is superior to other intracellular or external stimulus. UV light is a valid photon-controlled manner due to high efficiency. However, further applications of these approaches in living cells are hampered by the large dose of UV-light irradiation. To address this issue, a simultaneous light and host/guest mediation was proposed in this paper. Gold nanoparticles (AuNPs) encoding with mercapto-尾-cyclodextrin (尾CD) served as a carried agent. Azobenzene (Azo), which was labeled on a releasing oligonucleotide, acted as a photochemically controlled switch. Ferrocene (Fc), an excellent guest for inclusion complexation by 尾CD, serves as 鈥渆nhancers鈥?and shifts the equilibrium of the inclusion鈥揺xclusion process between trans-Azo and 尾CD under UV-light irradiation, thus making the dose of UV-light irradiation reduced obviously. For further application, transfected green fluorescent protein (GFP)-expressing human lung cancer A549 cells were used to determine cellular uptake and gene silencing mediated by our constructed system in vivo. The results demonstrate that by employing Fc host鈥揼uest interaction, about 62.4% gene silencing was achieved within 30 min, which is significantly higher than that without Fc competition. Our strategy provides the potential for orthogonal DNA delivery and therapeutic activation that would be capable of achieving higher levels of site-specific activity and reduced amounts of side effects.