Exploiting Fast Exciton Diffusion in Dye-Doped Polymer Nanoparticles to Engineer Efficient Photoswitching

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• 作者：Kateryna Trofymchuk ; Luca Prodi ; Andreas Reisch ; Yves M茅ly ; Kai Altenh枚ner ; Jochen Mattay ; Andrey S. Klymchenko
• 刊名：The Journal of Physical Chemistry Letters
• 出版年：2015
• 出版时间：June 18, 2015
• 年：2015
• 卷：6
• 期：12
• 页码：2259-2264
• 全文大小：316K
• ISSN：1948-7185

文摘

Photoswitching of bright fluorescent nanoparticles opens new possibilities for bioimaging with superior temporal and spatial resolution. However, efficient photoswitching of nanoparticles is hard to achieve using F枚rster resonance energy transfer (FRET) to a photochromic dye, because the particle size is usually larger than the F枚rster radius. Here, we propose to exploit the exciton diffusion within the FRET donor dyes to boost photoswitching efficiency in dye-doped polymer nanoparticles. To this end, we utilized bulky hydrophobic counterions that prevent self-quenching and favor communication of octadecyl rhodamine B dyes inside a polymer matrix of poly(mallcaps">d,mallcaps">l-lactide-co-glycolide). Among tested counterions, only perfluorinated tetraphenylborate that favors the exciton diffusion enables high photoswitching efficiency (on/off ratio 鈭?0). The switching improves with donor dye loading and requires only 0.1鈥?.3 wt % of a diphenylethene photochromic dye. Our nanoparticles were validated both in solution and at the single-particle level. The proposed concept paves the way to new efficient photoswitchable nanomaterials.