Multistage Regulator Based on Tandem Promoters and CRISPR/Cas

详细信息    查看全文

• 作者：Hangxing Jia ; Tong Liang ; Zhaoning Wang ; Zhaoren He ; Yang Liu ; Lei Yang ; Yan Zeng ; Shaopeng Liu ; Linyi Tang ; Jianbo Wang ; Yu Chen ; Zhixiong Xie
• 刊名：ACS Synthetic Biology
• 出版年：2014
• 出版时间：December 19, 2014
• 年：2014
• 卷：3
• 期：12
• 页码：1007-1010
• 全文大小：246K
• ISSN：2161-5063

文摘

Accurately controlling expression of target genes between several designed levels is essential for low-noise gene network and dynamic range of gene expression. However, such manipulations have been hard to achieve due to technical limitations. Based on tandem promoters and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) system, we constructed a multistage regulator that could stably regulate the expression of the reporter gene on three levels, with more than 2-fold difference between each of them. Our findings provide novel insights into constructing a more powerful gene regulation system.