Use of NMR Metabolomics To Analyze the Targets of D-Cycloserine in Mycobacteria: Role of D-Alanine Racemase

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• 作者：Steven Halouska ; Ofelia Chacon ; Robert J. Fenton ; Denise K. Zinniel ; Raul G. Barletta ; Robert Powers
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：December 2007
• 年：2007
• 卷：6
• 期：12
• 页码：4608 - 4614
• 全文大小：1046K
• 年卷期：v.6,no.12(December 2007)
• ISSN：1535-3907

文摘

D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.