A label-free colorimetric assay for detection of c-Myc mRNA based on peptide nucleic acid and silver nanoparticles

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• 作者：Xia Li ; Juan Song ; Bao-Li Chen ; Bing Wang ; Rui Li ; Hui-Min Jiang…
• 关键词：Colorimetric ; c ; Myc mRNA ; Peptide nucleic acid ; Silver nanoparticles ; 肽核酸
比色法 c ; Myc mRNA ; 纳米银
• 刊名：Chinese Science Bulletin
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：61
• 期：4
• 页码：276-281
• 全文大小：911 KB
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• 作者单位：Xia Li (1)
  Juan Song (1)
  Bao-Li Chen (1)
  Bing Wang (1)
  Rui Li (1)
  Hui-Min Jiang (1)
  Ji-Feng Liu (2)
  Chen-Zhong Li (1) (3)
  
  1. Department of Chemistry, Liaocheng University, Liaocheng, 252059, China
  2. Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China
  3. Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, 33174, USA
  
• 刊物主题：Science, general; Life Sciences, general; Physics, general; Chemistry/Food Science, general; Earth Sciences, general; Engineering, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-9541

文摘

A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles (PNA/AgNPs) has been initially proposed for specific recognition of mRNA. Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc mRNA detection can be achieved. Moreover, the PNA/AgNPs platform can undergo color change in response to target c-Myc mRNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes. Keywords Colorimetric c-Myc mRNA Peptide nucleic acid Silver nanoparticles