AFM characterization of chemically treated corneal cells

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• 作者：Teiko Shibata-Seki (1)
  Kazuki Tajima (2)
  Hiroki Takahashi (2)
  Hiroya Seki (3)
  Junji Masai (4)
  Hiroshi Goto (2)
  Eiry Kobatake (1)
  Toshihiro Akaike (5) (6)
  Norihiko Itoh (2) (7) (8)
  
  1. Department of Environmental Chemistry and Engineering ; Interdisciplinary Graduate School of Science and Engineering ; Tokyo Institute of Technology ; G1-13 4259 Nagatusta Midori-ku ; Yokohama ; 226-8502 ; Kanagawa ; Japan
  2. Tokyo Medical University ; 6-1-1 Shinjuku ; Shinjuku-ku ; Tokyo ; 160-8402 ; Japan
  3. Chemical Resources Laboratory ; Tokyo Institute of Technology ; R1-1 ; 4259 Nagatsuta ; Midori-ku ; Yokohama ; 226-8503 ; Japan
  4. Mitsubishi Chemical Techno-Research Corporation ; Yotsuya TN Bldg. 5F ; 16-1 Samoncho ; Shinjuku-ku ; Tokyo ; 160-0017 ; Japan
  5. Biomaterials Center for Regenerative Medical Engineering ; 1-1 Namiki ; Tsukuba ; Ibaraki ; 305-0044 ; Japan
  6. Graduate School of Bioscience and Biotechnology ; Tokyo Institute of Technology ; 4259 Nagatsuta-cho ; Midori-ku ; Yokohama ; 226-8501 ; Japan
  7. Tottori University Veterinary Medical Center ; 4-101 Koyama-Minami ; Tottori-shi ; 680-8553 ; Japan
  8. Dainichiseika-Donated Chair of Research Division for Innovative Biomaterials ; Center for Biological Resources and Informatics ; Tokyo Institute of Technology ; 4359-J3-615 ; Nagatsuta-cho ; Midori-ku ; Yokohama ; Japan
  
• 关键词：Atomic force microscopy ; Corneal epithelial and endothelial cells ; Mechanical property ; Young's modulus ; Glutaraldehyde
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：407
• 期：9
• 页码：2631-2635
• 全文大小：643 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

We present a characterization of chemically treated cells using atomic force microscopy (AFM) which can observe changes in morphology and elasticity of cells. Since AFM has the significant advantage that it does not require fixation of samples, the method is simple and can capture various properties of living cells. In this study, corneal epithelial and endothelial cells were examined. The topography images of the corneal cells without glutaraldehyde (GA) fixation were successfully obtained. The images showed a natural three-dimensional shape of these cells, which scanning electron microscope (SEM) images could not provide. The AFM images of GA-fixed cells were taken and compared with a SEM image reported in the literature. Our results show that longer time for GA fixation makes the surface of the corneal endothelial tissue stiffer. Also, longer treatment results in relatively large structural variation in samples. Combined with conventional histochemical methods, this approach helps us gain an overall understanding of the influence of such chemical treatment.