Genetic analysis and fine mapping of the LOBED-LEAF 1 (BnLL1) gene in rapeseed (Brassica napus L.)

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• 作者：Xiyuan Ni ; Jixiang Huang ; Basharat Ali ; Weijun Zhou ; Jianyi Zhao
• 关键词：Brassica napus ; Leaf margin ; LOBED ; LEAF 1 gene ; Fine mapping
• 刊名：Euphytica
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：204
• 期：1
• 页码：29-38
• 全文大小：1,738 KB
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• 作者单位：Xiyuan Ni (1) (2)
  Jixiang Huang (2)
  Basharat Ali (1)
  Weijun Zhou (1)
  Jianyi Zhao (2)
  
  1. Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China
  2. Institute of Crop Science and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5060

文摘

The lobed-leaf character is a clearly recognizable morphologic trait in rapeseed (Brassica napus L.) that has potential advantages in hybrid production. The present study was carried out to analyze the effect of lobed-leaf trait on agronomic traits and to fine map the LOBED-LEAF 1 (BnLL1) gene in B. napus. The lobed-leaf line Yuye 87 and entire-leaf cultivar Zheyou 50 were used to produce four F2 and eight BC1 populations for genetic analysis. Multiple comparison analysis between lobed-leaf character and agronomic traits was carried out in two F2 populations of reciprocal crosses between Yuye 87 and Zheyou 50. Results show that the lobed-leaf phenotype is controlled by a single an incomplete dominant gene and has no significant adverse effects on 10 main agronomic traits. The location of BnLL1 gene was narrowed down to a 36.7-kb homologous region of B. rapa between genes Bra009506 and Bra009511. On the basis of the annotation of orthologous genes in Arabidopsis, Bra009510 is considered to be a promising candidate for BnLL1 gene. The AT5G03790 is the orthologous gene of Bra009510 in Arabidopsis thaliana, with known functions in the formation of simple serrated leaves and in the suppression of bract formation. It is suggested that previously described lobed-leaf gene BnMOSAICLEAF (BnML) and the BnLL1 are the same genes controlling the lobed-leaf phenotype in B. napus, whereas the lobed-leaf gene BcLEAFLOBED 1 (BcLL1) is their homologue in Brassica campestris ssp. chinensis. These findings will facilitate the cloning and functional identification of the BnLL1 gene in future studies.