CRISPR/Cas9 and TALE: beyond cut and paste

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• 作者：Liping Deng (1)
  Ruotong Ren (1)
  Jun Wu (2)
  Keiichiro Suzuki (2)
  Juan Carlos Izpisua Belmote (2)
  Guang-Hui Liu (1) (3) (4)
  
  1. National Laboratory of Biomacromolecules ; Institute of Biophysics ; Chinese Academy of Sciences ; Beijing ; 100101 ; China
  2. Gene Expression Laboratory ; Salk Institute for Biological Studies ; 10010 North Torrey Pines Road ; La Jolla ; CA ; 92037 ; USA
  3. Beijing Institute for Brain Disorders ; Beijing ; 100069 ; China
  4. Center for Molecular and Translational Medicine ; CMTM ; Beijing ; 100101 ; China
  
• 关键词：TALE ; CRISPR/Cas9 ; genome editing ; gene expression ; transcription regulation
• 刊名：Protein & Cell
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：6
• 期：3
• 页码：157-159
• 全文大小：68 KB
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  5. Konermann, S (2013) Optical control of mammalian endogenous transcription and epigenetic states. Nature 500: pp. 472-476
  6. Konermann, S (2014) Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 512: pp. 441-444 CrossRef
  7. Li, M, Suzuki, K, Kim, NY, Liu, GH, Izpisua Belmonte, JC (2014) A cut above the rest: targeted genome editing technologies in human pluripotent stem cells. J Biol Chem 289: pp. 4594-4599 CrossRef
  8. Liu, GH (2014) Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCs. Nat Commun 5: pp. 4330
  9. Maeder, ML (2013) Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Nat Biotechnol 31: pp. 1137-1142 CrossRef
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  13. Pan, H, Zhang, W, Liu, GH (2011) Find and replace: editing human genome in pluripotent stem cells. Protein Cell 2: pp. 950-956 CrossRef
  14. Smith, C (2014) Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs. Cell Stem Cell 15: pp. 12-13 CrossRef
  15. Suzuki, K (2014) Targeted gene correction minimally impacts whole-genome mutational load in human-disease-specific induced pluripotent stem cell clones. Cell Stem Cell 15: pp. 31-36 CrossRef
  16. Therizols, P (2014) Chromatin decondensation is sufficient to alter nuclear organization in embryonic stem cells. Science 346: pp. 1238-1242 CrossRef
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  18. Zalatan JG et al (2014) Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds. Cell 160:339鈥?50
  
• 刊物主题：Biochemistry, general; Protein Science; Cell Biology; Stem Cells; Human Genetics; Developmental Biology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1674-8018

文摘

Nuclease-based genome editing has proven to be a powerful and promising tool for disease modeling and gene therapy. Recent advances in CRISPR/Cas and TALE indicate that they could also be used as a targeted regulator of gene expression, as well as being utilized for illuminating specific chromosomal structures or genomic regions.