9-Oxo-10(E),12(Z),15(Z)-Octadecatrienoic Acid Activates Peroxisome Proliferator-Activated Receptor α in Hepatocytes

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• 作者：Haruya Takahashi ; Kosuke Kamakari ; Tsuyoshi Goto ; Hideyuki Hara ; Shinsuke Mohri…
• 关键词：PPARα ; Oxylipin ; Hepatocytes ; Fatty acid metabolism ; LC–MS
• 刊名：Lipids
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：50
• 期：11
• 页码：1083-1091
• 全文大小：635 KB
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• 作者单位：Haruya Takahashi (1)
  Kosuke Kamakari (1)
  Tsuyoshi Goto (1) (2)
  Hideyuki Hara (1)
  Shinsuke Mohri (1)
  Hideyuki Suzuki (3)
  Daisuke Shibata (3)
  Rieko Nakata (4)
  Hiroyasu Inoue (4)
  Nobuyuki Takahashi (1) (2)
  Teruo Kawada (1) (2)
  
  1. Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 611-0011, Japan
  2. Research Unit for Physiological Chemistry, Kyoto University, Kyoto, 606-8501, Japan
  3. Kazusa DNA Research Institute, Chiba, 292-0818, Japan
  4. Department of Food Science and Nutrition, Nara Women’s University, Nara, 630-8506, Japan
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Life Sciences
  Biochemistry
  Medicinal Chemistry
  Microbial Genetics and Genomics
  Nutrition
  Bioorganic Chemistry
  Medical Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1558-9307

文摘

The peroxisome proliferator-activated receptor (PPAR)α is mainly expressed in the liver and plays an important role in the regulation of lipid metabolism. It has been reported that PPARα activation enhances fatty acid oxidation and reduces fat storage. Therefore, PPARα agonists are used to treat dyslipidemia. In the present study, we found that 9-oxo-10(E),12(Z),15(Z)-octadecatrienoic acid (9-oxo-OTA), which is a α-linolenic acid (ALA) derivative, is present in tomato (Solanum lycopersicum) extract. We showed that 9-oxo-OTA activated PPARα and induced the mRNA expression of PPARα target genes in murine primary hepatocytes. These effects promoted fatty acid uptake and the secretion of β-hydroxybutyrate, which is one of the endogenous ketone bodies. We also demonstrated that these effects of 9-oxo-OTA were not observed in PPARα-knockout (KO) primary hepatocytes. To our knowledge, this is the first study to report that 9-oxo-OTA promotes fatty acid metabolism via PPARα activation and discuss its potential as a valuable food-derived compound for use in the management of dyslipidemia. Keywords PPARα Oxylipin Hepatocytes Fatty acid metabolism LC–MS