Validation of A multiplex PCR detection kit for screening of herbicide-tolerant genes in genetically modified crops

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• 作者：Jae-Hwan Kim (13229)
  Eun-Hee Kim (23229)
  Mi-Chi Yea (33229)
  Hae-Yeong Kim (13229)
  
• 关键词：detection kit ; genetically modified crops ; herbicide tolerance ; multiplex polymerase chain reaction ; screening
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：56
• 期：2
• 页码：251-254
• 全文大小：303KB
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  2. Bahrdt C, Krech AB, Wurz A, and Wulff D (2010) Validation of a newly developed hexaplex real-time PCR assay for screening for presence of GMOs in food, feed and seed. / Anal Bioanal Chem 396, 2103鈥?112. CrossRef
  3. Dinon AZ, Prins TW, van Dijk JP, Arisi AC, Scholtens IM, and Kok EJ. (2011) Development and validation of real-time PCR screening methods for detection of cry1A.105 and cry2Ab2 genes in genetically modified organisms. / Anal Bioanal Chem 400, 1433鈥?442. CrossRef
  4. D枚rries HH, Remus I, Gr枚newald A, Gr枚newald C, and Berghof-J盲ger K (2010) Development of a qualitative, multiplex real-time PCR kit for screening of genetically modified organisms (GMOs). / Anal Bioanal Chem 396, 2043鈥?054. CrossRef
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  11. Kim JH, Kim TW, Lee WY, Park SH, and Kim HY (2007) Multiplex PCR detection of the GT73, MS8xRF3, and T45 varieties of GM canola. / Food Sci Biotechnol 16, 104鈥?09.
  12. Kim JH, Kim SA, Seo YJ, Lee WY, Park SH, and Kim HY (2008) Multiplex PCR detection of the MON1445, MON15985, MON88913, and LLcotton25 varieties of GM cotton. / Food Sci Biotechnol 17, 829鈥?32.
  13. Kim JH, Seo YJ, Sun SH, and Kim HY (2009) Multiplex PCR detection of 4 events of genetically modified soybean (RRS, A2704-12, DP356043-5, and MON89788). / Food Sci Biotechnol 18, 694鈥?99.
  
• 作者单位：Jae-Hwan Kim (13229)
  Eun-Hee Kim (23229)
  Mi-Chi Yea (33229)
  Hae-Yeong Kim (13229)
  
  13229. Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea
  23229. PLUTOS Incorporated, Seoul, 135-565, Republic of Korea
  33229. Department of Plant Quarantine, Animal, Plant and Fisheries Quarantine and Inspection Agency, 433-1 Anyang, 430-016, Republic of Korea
  
• ISSN：2234-344X

文摘

A multiplex polymerase chain reaction (PCR) detection kit for screening of four herbicide-tolerant genes (cp4 epsps, mepsps, pat, and bar) in genetically modified (GM) crops was developed. The kit was validated by three different laboratories, and the expected targets were specifically observed in 14 different herbicide-tolerant GM events. This method can be effectively and conveniently used to monitor approved and unapproved GM crops containing four herbicide-tolerant genes.