Backbone and side-chain 1H, 15N, 13C assignment and secondary structure of BPSL1445 from Burkholderia pseudomallei

详细信息    查看全文

• 作者：Giacomo Quilici ; Andrea Berardi ; Davide Gaudesi…
• 关键词：Melioidosis ; Infectious diseases ; Host ; pathogen interactions ; Vaccine ; Biological warfare agent
• 刊名：Biomolecular NMR Assignments
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：9
• 期：2
• 页码：347-350
• 全文大小：515 KB
• 参考文献：Aldhous P (2005) Tropical medicine: melioidosis? Never heard of it. Nature 434(7034):692鈥?93CrossRef ADS
  Cheng AC, Currie BJ (2005) Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18(2):383鈥?16CrossRef
  Currie BJ, Dance DA, Cheng AC (2008) The global distribution of Burkholderia pseudomallei and melioidosis: an update. Trans R Soc Trop Med Hyg 102(Suppl 1):S1鈥揝4CrossRef
  Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6(3):277鈥?93CrossRef
  Dowling AJ (2013) Novel gain of function approaches for vaccine candidate identification in Burkholderia pseudomallei. Front Cell Infect Microbiol 2:139CrossRef
  Felgner PL, Kayala MA, Vigil A, Burk C, Nakajima-Sasaki R, Pablo J, Molina DM, Hirst S, Chew JS, Wang D et al (2009) A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens. Proc Natl Acad Sci U S A 106(32):13499鈥?3504CrossRef ADS
  Finco O, Rappuoli R (2014) Designing Vaccines for the Twenty-First Century Society. Front Immunol 5:12CrossRef
  Hafsa NE, Wishart DS (2014) CSI 2.0鈥攁 significantly improved version of the Chemical Shift Index. J Biomol NMR 60(2鈥?):131鈥?46CrossRef
  Hasegawa J, Tokuda E, Tenno T, Tsujita K, Sawai H, Hiroaki H, Takenawa T, Itoh T (2011) SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain. J Cell Biol 193(5):901鈥?16CrossRef
  Petersen TN, Brunak S, von Heijne G, Nielsen H (2011) SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods 8(10):785鈥?86CrossRef
  Sarkar-Tyson M, Titball RW (2010) Progress toward development of vaccines against melioidosis: a review. Clin Ther 32(8):1437鈥?445CrossRef
  Sattler M, Schleucherb J, Griesinger C (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog Nucl Magn Reson Spectrosc 34:93鈥?58CrossRef
  Sklenar V, Brooks B, Zon G, Bax A (1987) Absorption mode two-dimensional NOE spectroscopy of exchangeable protons in oligonucleotides. FEBS Lett 216:249鈥?52CrossRef
  Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59(4):687鈥?96CrossRef
  Wiersinga WJ, van der Poll T, White NJ, Day NP, Peacock SJ (2006) Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei. Nat Rev Microbiol 4(4):272鈥?82CrossRef
  
• 作者单位：Giacomo Quilici (1)
  Andrea Berardi (1)
  Davide Gaudesi (1)
  Louise J. Gourlay (2)
  Martino Bolognesi (2)
  Giovanna Musco (1)
  
  1. Biomolecular NMR Unit, c/o S. Raffaele Scientific Institute, Via Olgettina, 58, Milan, Italy
  2. Department of Biosciences, University of Milan, Via Celoria, 26, 20133, Milan, Italy
  
• 刊物类别：Physics and Astronomy
• 刊物主题：None Assigned
• 出版者：Springer Netherlands
• ISSN：1874-270X

文摘

BPSL1445 is a lipoprotein produced by the Gram-negative bacterium Burkholderia pseudomallei (B. pseudomallei), the etiological agent of melioidosis. Immunodetection assays against sera patients using protein microarray suggest BPSL1445 involvement in melioidosis. Herein we report backbone, side chain NMR assignment and secondary structure for the recombinant protein. Keywords Melioidosis Infectious diseases Host-pathogen interactions Vaccine Biological warfare agent