Application of carboxyphenylboronic acid-functionalized magnetic nanoparticles for extracting nucleic acid from seeds

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• 作者：Ning Sun (1) (2) (3) (4)
  Congliang Deng (3)
  Guanglu Ge (4)
  Qiang Xia (1) (2)
  
  1. State Key Laboratory of Bioelectronics ; School of Biological Science and Medical Engineering ; Southeast University ; Sipailou No. 2 ; Nanjing ; 210096 ; China
  2. Collaborative Innovation Center of Suzhou Nano Science and Technology ; Suzhou ; 215123 ; China
  3. Beijing Entry-exit Inspection and Quarantine Bureau ; Beijing ; 100026 ; China
  4. CAS Key Laboratory of Measurement and Standardization for Nanotechnology ; National Center for Nanoscience and Technology ; Beijing ; 100190 ; China
  
• 关键词：4 ; Carboxyphenylboronic acid ; DNA extraction ; Magnetic nanoparticles ; Maize seeds ; RNA extraction ; Seed ; borne virus ; Soybean seeds
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：37
• 期：1
• 页码：211-218
• 全文大小：1,961 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Magnetic iron oxide nanoparticles functionalized with 4-carboxyphenylboronic acid (CPBA-MNPs) were developed for extracting genomic DNA, total RNA and nucleic acids from seeds. The seed samples were genetically-modified maize seeds and unmodified soybean seeds infected by bean pod mottle virus and tobacco ringspot virus. The total nucleic acids, genomic DNA, and RNA could be separately extracted from these seeds with high qualities using CPBA-MNPs under different conditions. Furthermore, the results of real-time quantitative qPCR and real-time reverse transcription (RT)-PCR indicated that the nucleic acids extracted from these seeds using CPBA-MNPs were suitable for the detection of genetically-modified seeds and seed-borne viruses.