miR-302b is a potential molecular marker of esophageal squamous cell carcinoma and functions as a tumor suppressor by targeting ErbB4

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• 作者：Mingxin Zhang (7)
  Qi Yang (7)
  Lingmin Zhang (8)
  Suna Zhou (6)
  Wenguang Ye (7)
  Qinglin Yao (7)
  Zongfang Li (7)
  Cheng Huang (8)
  Qinsheng Wen (7)
  Jingjie Wang (7)
  
• 关键词：miR ; 302b ; ErbB4 ; Esophageal squamous cell carcinoma
• 刊名：Journal of Experimental & Clinical Cancer Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：33
• 期：1
• 全文大小：1,168 KB
• 作者单位：Mingxin Zhang (7)
  Qi Yang (7)
  Lingmin Zhang (8)
  Suna Zhou (6)
  Wenguang Ye (7)
  Qinglin Yao (7)
  Zongfang Li (7)
  Cheng Huang (8)
  Qinsheng Wen (7)
  Jingjie Wang (7)
  
  7. Department of General Surgery, Second Affiliated Hospital, School of Medicine, Xi鈥檃n Jiaotong University, Xi鈥檃n, 710004, Shaanxi Province, China
  8. Department of genetics and molecular biology, Medical school, Xi鈥檃n Jiaotong University/Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi鈥檃n, 710061, Shaanxi Province, China
  6. Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi鈥檃n, 710038, Shaanxi Province, China
  
• ISSN：1756-9966

文摘

Background ErbB4 expression has been noted in various tumors, but its regulatory mechanism in esophageal squamous cell carcinoma (ESCC) remains unclear. The aim of this study was to investigate whether miR-302b regulates the expression of ErbB4 at the post-transcriptional level and to determine its expression, significance, and function in ESCC. Methods We used real-time reverse transcriptase-polymerase chain reaction to quantify the expression of miR-302b in 50 ESCC tissues and analyzed its relationship with clinicopathological factors and survival. Then, we investigated the post-transcriptional regulation of ErbB4 expression using immunoblot analysis and luciferase reporter assays. Finally, the effects of miR-302b on proliferation, apoptosis, and invasion of ESCC cells was detected using MTT, flow cytometric analysis, and transwell invasion assays, respectively. Results miR-302b was significantly down-regulated and correlated with tumor differentiation and lymph node metastasis in ESCC. Univariate and multivariate analyses indicated that low miR-302b expression might be a poor prognostic factor. Further studies demonstrated that miR-302b post-transcriptionally down-regulated the expression of ErbB4 in vitro. Moreover, miR-302b inhibited proliferation by inducing apoptosis and repressed invasion in the ESCC cell lines. Conclusions miR-302b is a potential molecular marker of ESCC and functions as a tumor suppressor by post-transcriptionally regulating ErbB4.