miRNAs promote generation of porcine-induced pluripotent stem cells

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• 作者：Kuiying Ma (1)
  Guangqi Song (1)
  Xinglan An (1)
  Anran Fan (1)
  Wentao Tan (1)
  Bo Tang (1)
  Xueming Zhang (1)
  Ziyi Li (1)
  
• 关键词：Porcine ; induced pluripotent stem cells (piPSCs) ; microRNA
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：389
• 期：1-2
• 页码：209-218
• 全文大小：11,720 KB
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• 作者单位：Kuiying Ma (1)
  Guangqi Song (1)
  Xinglan An (1)
  Anran Fan (1)
  Wentao Tan (1)
  Bo Tang (1)
  Xueming Zhang (1)
  Ziyi Li (1)
  
  1. Jilin Provincial Key Laboratory of Animal Embryo Engineering, The Center for Animal Embryo Engineering of Jilin Province, College of Veterinary Medicine, Jilin University, 5333 Xi An Da Lu, Changchun, 130062, Jilin, China
  
• ISSN：1573-4919

文摘

The pigs have similarities of organ size, immunology and physiology with humans. Porcine-induced pluripotent stem cells (piPSCs) have great potential application in regenerative medicine. Here, we established piPSCs induced from porcine fetal fibroblasts by the retroviral overexpression of Oct4, Sox2, Klf4, and c-Myc. The piPSCs not only express pluripotent markers but also have the capacity for differentiation in vivo and in vitro, including EB and teratoma formation. We supplemented microRNAs during the induction process because miR-302a, miR-302b, and miR-200c have been reported to be highly expressed in human and mouse embryonic stem cells and in iPSCs. In this study, we found that the overexpression of miR-302a, miR-302b, and miR-200c effectively improved the reprogramming efficiency and reduced the induction time for piPSCs in the OSKM and OSK induction systems. Due to the similar induction efficiency of 4F-induced piPSCs or of three factors combined with miR-302a, miR-302b, and miR-200c (3F-miRNA-induced piPSCs), we recommend the addition of miRNAs instead of c-Myc to reduce the tumorigenicity of piPSCs.