Down-regulated aquaporin 5 inhibits proliferation and migration of human epithelial ovarian cancer 3AO cells

详细信息    查看全文

• 作者：ChunXiao Yan (1)
  Yunshan Zhu (2)
  Xiao Zhang (2)
  Xuejun Chen (1)
  Wei Zheng (1)
  Jianhua Yang (2)
  
  1. Department of Gynecology ; The Second Affiliated Hospital ; School of Medicine ; Zhejiang University ; Hangzhou ; Zhejiang Province ; China
  2. Department of Obstetrics & Gynecology ; Sir Run Run Shaw Hospital affiliated to the Zhejiang University School of Medicine ; Hangzhou ; Zhejiang Province ; 310016 ; China
  
• 关键词：3AO cell ; Aquaporins 5 ; Migration ; Proliferation ; Tumorigenesis
• 刊名：Journal of Ovarian Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：3,100 KB
• 参考文献：1. Siegel, R, Ma, J, Zou, Z, Jemal, A (2014) Cancer statistics, 2014. CA Cancer J Clin 64: pp. 9-29 CrossRef
  2. Su, Z, Graybill, WS, Zhu, Y (2013) Detection and monitoring of ovarian cancer. Clin Chim Acta 415: pp. 341-345 CrossRef
  3. Yurkovetsky, Z, Skates, S, Lomakin, A, Nolen, B, Pulsipher, T, Modugno, F, Marks, J, Godwin, A, Gorelik, E, Jacobs, I, Menon, U, Lu, K, Badgwell, D, Bast, RC, Lokshin, AE (2010) Development of a multimarker assay for early detection of ovarian cancer. J Clin Oncol 28: pp. 2159-2166 CrossRef
  4. Ko, SY, Naora, H (2014) Therapeutic strategies for targeting the ovarian tumor stroma. World J Clin Cases 2: pp. 194-200
  5. Jeyaseelan, K, Sepramaniam, S, Armugam, A, Wintour, EM (2006) Aquaporins: a promising target for drug development. Expert Opin Ther Targets 10: pp. 889-909 CrossRef
  6. Hachez, C, Chaumont, F (2010) Aquaporins: a family of highly regulated multifunctional channels. Adv Exp Med Biol 679: pp. 1-17 CrossRef
  7. Ishibashi, K, Hara, S, Kondo, S (2009) Aquaporin water channels in mammals. Clin Exp Nephrol 13: pp. 107-117 CrossRef
  8. Schey, KL, Wang, Z, LWenke, J, Qi, Y (2014) Aquaporins in the eye: expression, function, and roles in ocular disease. Biochim Biophys Acta 1840: pp. 1513-1523 CrossRef
  9. Ribatti, D, Ranieri, G, Annese, T, Nico, B (2014) Aquaporins in cancer. Biochim Biophys Acta 1840: pp. 1550-1553 CrossRef
  10. Holmes, RP (2012) The role of renal water channels in health and disease. Mol Aspects Med 33: pp. 547-552 CrossRef
  11. Huber, VJ, Tsujita, M, Nakada, T (2012) Aquaporins in drug discovery and pharmacotherapy. Mol Aspects Med 33: pp. 691-703 CrossRef
  12. Wang, F, Feng, XC, Li, YM, Yang, H, Ma, TH (2006) Aquaporins as potential drug targets. Acta Pharmacol Sin 27: pp. 395-401 CrossRef
  13. Raina, S, Preston, GM, Guggino, WB, Agre, P (1995) Molecular cloning and characterization of an aquaporin cDNA from salivary, lacrimal, and respiratory tissues. J Biol Chem 270: pp. 1908-1912 CrossRef
  14. Funaki, H, Yamamoto, T, Koyama, Y, Kondo, D, Yaoita, E, Kawasaki, K, Kobayashi, H, Sawaguchi, S, Abe, H, Kihara, I (1998) Localization and expression of AQP5 in cornea, serous salivary glands, and pulmonary epithelial cells. Am J Physiol 275: pp. C1151-1157
  15. Yang, JH, Shi, YF, Cheng, Q, Deng, L (2006) Expression and localization of aquaporin-5 in the epithelial ovarian tumors. Gynecol Oncol 100: pp. 294-299 CrossRef
  16. Chae, YK, Woo, J, Kim, MJ, Kang, SK, Kim, MS, Lee, J, Lee, SK, Gong, G, Kim, YH, Soria, JC, Jang, SJ, Sidransky, D, Moon, C (2008) Expression of aquaporin 5 (AQP5) promotes tumor invasion in human non small cell lung cancer. PLoS One 3: pp. e2162 CrossRef
  17. Shi, Z, Zhang, T, Luo, L, Zhao, H, Cheng, J, Xiang, J, Zhao, C (2012) Aquaporins in human breast cancer: identification and involvement in carcinogenesis of breast cancer. J Surg Oncol 106: pp. 267-272 CrossRef
  18. Wang, W, Li, Q, Yang, T, Bai, G, Li, D, Sun, H (2012) Expression of AQP5 and AQP8 in human colorectal carcinoma and their clinical significance. World J Surg Oncol 10: pp. 242 CrossRef
  19. Huang, YH, Zhou, XY, Wang, HM, Xu, H, Chen, J, Lv, NH (2013) Aquaporin 5 promotes the proliferation and migration of human gastric carcinoma cells. Tumour Biol 34: pp. 1743-1751 CrossRef
  20. Woo, J, Lee, J, Chae, YK, Kim, MS, Baek, JH, Park, JC, Park, MJ, Smith, IM, Trink, B, Ratovitski, E, Lee, T, Park, B, Jang, SJ, Soria, JC, Califano, JA, Sadransky, D, Moon, C (2008) Overexpression of AQP5, a putative oncogene, promotes cell growth and transformation. Cancer Lett 264: pp. 54-62 CrossRef
  21. Zhang, Z, Chen, Z, Song, Y, Zhang, P, Hu, J, Bai, C (2010) Expression of aquaporin 5 increases proliferation and metastasis potential of lung cancer. J Pathol 221: pp. 210-220 CrossRef
  22. Jung, HJ, Park, JY, Jeon, HS, Kwon, TH (2011) Aquaporin-5: a marker protein for proliferation and migration of human breast cancer cells. PLoS One 6: pp. e28492 CrossRef
  23. Jiang, XX, Xu, KH, Ma, JY, Tian, YH, Guo, XY, Lin, J, Wu, RJ (2012) Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5. Oncol Lett 4: pp. 257-261
  24. Woo, J, Lee, J, Kim, MS, Jang, SJ, Sidransky, D, Moon, C (2008) The effect of aquaporin 5 overexpression on the Ras signaling pathway. Biochem Biophys Res Commun 367: pp. 291-298 CrossRef
  25. Yang, J, Yan, C, Zheng, W, Chen, X (2012) Proliferation inhibition of cisplatin and aquaporin 5 expression in human ovarian cancer cell CAOV3. Arch Gynecol Obstet 285: pp. 239-245 CrossRef
  26. Yang, JH, Yu, YQ, Yan, CX (2011) Localisation and expression of aquaporin subtypes in epithelial ovarian tumours. Histol Histopathol 26: pp. 1197-1205
  27. Shen, L, Zhu, Z, Huang, Y, Shu, Y, Sun, M, Xu, H, Zhang, G, Guo, R, Wei, W, Wu, W (2010) Expression profile of multiple aquaporins in human gastric carcinoma and its clinical significance. Biomed Pharmacother 64: pp. 313-318 CrossRef
  28. Frede, J, Fraser, SP, Oskay-Ozcelik, G, Hong, Y, Ioana Braicu, E, Sehouli, J, Gabra, H, Djamgoz, MB (2013) Ovarian cancer: Ion channel and aquaporin expression as novel targets of clinical potential. Eur J Cancer 49: pp. 2331-2344 CrossRef
  29. Chen, Z, Zhang, Z, Gu, Y, Bai, C (2011) Impaired migration and cell volume regulation in aquaporin 5-deficient SPC-A1 cells. Respir Physiol Neurobiol 176: pp. 110-117 CrossRef
  
• 刊物主题：Gynecology; Reproductive Medicine;
• 出版者：BioMed Central
• ISSN：1757-2215

文摘

Background Recent studies suggested that aquaporins 5 (AQP5) was associated with many kinds of cancers and regulated many processes of various kinds of cancer cells. Our previous studies also demonstrated that AQP5 was highly expressed in epithelial ovarian cancer and contributed to the progress of ovarian cancer. Methods Lentivirus for knocking-down the expression of AQP5 was prepared and verified by qPCR and Western blotting. Cell counting kit-8 (CCK8) assay and transwell assay were performed to investigate the role of AQP5 on proliferation and migration of 3AO cells. The effects of down-regulating AQP5 on tumorigenesis were tested by tumor xenografts experiments. Results An effective lentivirus silencing AQP5 expression was obtained and used in this study. Down-regulating AQP5 inhibited proliferation and migration of cultured human epithelial ovarian cancer 3AO Cell. Furthermore, interfering of AQP5 during tumorigenesis could efficiently decrease the tumor growth in athymic mice. Conclusions These findings altogether suggest that AQP5 regulated multi processes in ovarian carcinogenesis and may be an attractive therapeutic target.