Molecular Genotyping of Herbicide Resistance in P. minor: ACCase Resistance

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• 作者：Rajender Singh (1)
  Davinder Sharma (1)
  Nishu Raghav (1)
  Rajender Singh Chhokar (1)
  Indu Sharma (1)
  
  1. ICAR - Directorate of Wheat Research ; Karnal ; 132001 ; India
  
• 关键词：Herbicide resistance ; SSCP ; Mutation ; Acetyl ; CoA carboxylase ; Phalaris minor
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：175
• 期：3
• 页码：1617-1621
• 全文大小：479 KB
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  2. Chhokar, R. S., & Sharma, R. K. (2008) Multiple herbicide resistance in littleseed canarygrass ( / Phalaris minor): a threat to wheat production in India. / Weed Biology and Management, 8, 112鈥?23.
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

Little seed canary grass (Phalaris minor Retz.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase) represent an increasingly important weed control problem in northern India. The objective of this study was to develop DNA-based markers to differentiate herbicide-resistant and herbicide-susceptible population of P. minor. Primers were designed to amplify the conserved region carrying two reported mutations Trp2027 to Cys and Ile2041 to Asn conferring ACCase inhibitor resistance in several grass weeds and subjected to single-strand conformational polymorphism (SSCP) to detect the mutations. Five distinctive electrophoretic patterns on non-denaturing PAGE were observed, and four patterns were found to be associated with ACCase herbicide resistance in P. minor. The PCR-SSCP test developed in this study confirmed 17 resistant populations to contain mutations in CT domain of ACCase gene. This is the first report of rapid and easy molecular diagnosis of ACCase herbicide-resistant and herbicide-sensitive population of P. minor through PCR-SSCP analysis.