Preparation of (3R, 3′R)-astaxanthin monoester and (3R, 3′R)-astaxanthin from Antarctic krill (Euphausia superba Dana)

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• 作者：Shasha Zhang ; Xiaoyun Sun ; Daicheng Liu
• 关键词：(3R ; 3′R) ; Astaxanthin monoester ; (3R ; 3′R) ; Astaxanthin ; Antarctic krill (Euphausia superba Dana) ; HPLC ; Silica gel column chromatography ; GC–MS
• 刊名：European Food Research and Technology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：240
• 期：2
• 页码：295-299
• 全文大小：353 KB
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• 作者单位：Shasha Zhang (1)
  Xiaoyun Sun (1)
  Daicheng Liu (1)
  
  1. College of Life Science, Shandong Normal University, 88 East Wenhua Road, Jinan, 250014, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Analytical Chemistry
  Biotechnology
  Agriculture
  Forestry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-2385

文摘

A special method is described for the preparation of (3R, 3′R)-astaxanthin monoester (AM) and (3R, 3′R)-astaxanthin from Antarctic krill (Euphausia superba Dana). Purified AM was obtained on a large scale from crude extract extracted from Antarctic krill in 38.14?% yield by silica gel column chromatography. After the AM was saponified with methanolic KOH (0.10?mol?L?) and separated by high-performance thin-layer chromatography, pure astaxanthin was obtained. There was only one optical (3R, 3′R)-isomer of the esterification of astaxanthin with (-)-camphanic acid chloride detected by high-performance liquid chromatography compared with astaxanthin standard. It was further proved that the AM consists of only one optical (3R, 3′R)-isomer. Twelve types of fatty acids in the AM from Antarctic krill were found by gas chromatography–mass spectrometer. Eicosapentaenoic and docosahexaenoic acids are the major fatty acids. It is the first time to prepare (3R, 3′R)-AM and (3R, 3′R)-astaxanthin from Antarctic krill. The purified (3R, 3′R)-AM and (3R, 3′R)-astaxanthin can be used as a standard for further related study.