Freshly frozen E18 rat cortical cells can generate functional neural networks after standard cryopreservation and thawing procedures

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• 作者：Kim Quasthoff (1)
  Stefano Ferrea (1) (3)
  Wiebke Fleischer (3)
  Stephan Theiss (3)
  Alfons Schnitzler (3)
  Marcel Dihn茅 (2)
  Janine Walter (1) (2)
  
  1. Department of Neurology ; Medical Faculty ; Heinrich-Heine University ; Moorenstr. 5 ; 40225 ; D眉sseldorf ; Germany
  3. Medical Faculty ; Institute of Clinical Neuroscience and Medical Psychology ; Heinrich-Heine University ; Universit盲tsstr. 1 ; 40225 ; D眉sseldorf ; Germany
  2. Department of Neurology and Epileptology ; Hertie Institute for Clinical Brain Research ; Eberhard-Karls-University ; Hoppe-Seyler-Stra脽e 3 ; 72076 ; T眉bingen ; Germany
  
• 关键词：Primary rat E18 cortical neurons ; Cryopreservation of cells ; In vitro ; neuronal network activity (ivNNA)
• 刊名：Cytotechnology
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：67
• 期：3
• 页码：419-426
• 全文大小：5,914 KB
• 参考文献：1. Ballarin, C, Peruffo, A (2012) Primary cultures of astrocytes from fetal bovine brain. Methods Mol Biol 814: pp. 117-126 CrossRef
  2. Berdondini, L, Massobrio, P, Chiappalone, M, Tedesco, M, Imfeld, K, Maccione, A, Gandolfo, M, Koudelka-Hep, M, Martinoia, S (2009) Extracellular recordings from locally dense microelectrode arrays coupled to dissociated cortical cultures. J Neurosci Methods 177: pp. 386-396 CrossRef
  3. Chiappalone, M, Bove, M, Vato, A, Tedesco, M, Martinoia, S (2006) Dissociated cortical networks show spontaneously correlated activity patterns during in vitro development. Brain Res 1093: pp. 41-53 CrossRef
  4. Das, GD, Houle, JD, Brasko, J, Das, KG (1983) Freezing of neural tissues and their transplantation in the brain of rats: technical details and histological observations. J Neurosci Methods 8: pp. 1-15 CrossRef
  5. Drayton, S, Peters, G (2002) Immortalisation and transformation revisited. Curr Opin Genet Dev 12: pp. 98-104 CrossRef
  6. Fang, J, Zhang, ZX (1992) Cryopreservation of embryonic cerebral tissue of rat. Cryobiology 29: pp. 267-273 CrossRef
  7. Giordano, G, Costa, LG (2011) Primary neurons in culture and neuronal cell lines for in vitro neurotoxicological studies. Methods Mol Biol 758: pp. 13-27 CrossRef
  8. Gonos, ES, Spandidos, DA (1993) Oncogenes in cellular immortalisation and differentiation (review). Anticancer Res 13: pp. 1117-1122
  9. Grout, B, Morris, J, McLellan, M (1990) Cryopreservation and the maintenance of cell lines. Trends Biotechnol 8: pp. 293-297 CrossRef
  10. Grumet, AE, Wyatt, JL, Rizzo, JF (2000) Multi-electrode stimulation and recording in the isolated retina. J Neurosci Methods 101: pp. 31-42 CrossRef
  11. Heuschkel, MO, Fejtl, M, Raggenbass, M, Bertrand, D, Renaud, P (2002) A three-dimensional multi-electrode array for multi-site stimulation and recording in acute brain slices. J Neurosci Methods 114: pp. 135-148 CrossRef
  12. Illes, S, Theiss, S, Hartung, HP, Siebler, M, Dihn茅, M (2009) Niche-dependent development of functional neuronal networks from embryonic stem cell-derived neural populations. BMC Neurosci 10: pp. 93 CrossRef
  13. Ivenshitz, M, Segal, M (2010) Neuronal density determines network connectivity and spontaneous activity in cultured hippocampus. J Neurophysiol 104: pp. 1052-1060 CrossRef
  14. Jensen, S, Sorensen, T, Zimmer, J (1987) Cryopreservation of fetal rat brain tissue later used for intracerebral transplantation. Cryobiology 24: pp. 120-134 CrossRef
  15. Jolly, CN, Clopton, BM, Spelman, FA, Lineaweaver, SK (1997) Guinea pig auditory nerve response triggered by a high density electrode array. Med Prog Technol 21: pp. 13-23
  16. Mattson, MP, Rychlik, B (1990) Cell culture of cryo-preserved human fetal cerebral cortical and hippocampal neurons: neuronal development and responses to trophic factors. Brain Res 522: pp. 204-214 CrossRef
  17. Milosevic, J, Storch, A, Schwarz, J (2005) Cryopreservation does not affect proliferation and multipotency of murine neural precursor cells. Stem Cells 23: pp. 681-688 CrossRef
  18. Morris, CB (2007) Cryopreservation of animal and human cell lines. Methods Mol Biol 368: pp. 227-236 CrossRef
  19. Muldrew, K, McGann, LE (1990) Mechanisms of intracellular ice formation. Biophys J 57: pp. 525-532 CrossRef
  20. Muldrew, K, McGann, LE (1994) The osmotic rupture hypothesis of intracellular freezing injury. Biophys J 66: pp. 532-541 CrossRef
  21. Negishi, T, Ishii, Y, Kawamura, S, Kuroda, Y, Yoshikawa, Y (2002) Cryopreservation of brain tissue for primary culture. Exp Anim 51: pp. 383-390 CrossRef
  22. Negishi T, Ishii Y, Kyuwa S, Kuroda Y, Yoshikawa Y (2003) Primary culture of cortical neurons, type-1 astrocytes, and microglial cells from cynomolgus monkey ( / Macaca fascicularis) fetuses. J Neurosci Methods 131:133鈥?40
  23. Novellino, A, Scelfo, B, Palosaari, T, Price, A, Sobanski, T, Shafer, TJ, Johnstone, AF, Gross, GW, Gramowski, A, Schroeder, O, Jugelt, K, Chiappalone, M, Benfenati, F, Martinoia, S, Tedesco, MT, Defranchi, E, D鈥橝ngelo, P, Whelan, M (2011) Development of micro-electrode array based tests for neurotoxicity: assessment of interlaboratory reproducibility with neuroactive chemicals. Front Neuroeng 4: pp. 4 CrossRef
  24. Odell, A, Askham, J, Whibley, C, Hollstein, M (2010) How to become immortal: let MEFs count the ways. Aging (Albany NY) 2: pp. 160-165
  25. Otto, F, Gortz, P, Fleischer, W, Siebler, M (2003) Cryo-preserved rat cortical cells develop functional neuronal networks on microelectrode arrays. J Neurosci Methods 128: pp. 173-181 CrossRef
  26. Paynter, SJ (2008) Principles and practical issues for cryopreservation of nerve cells. Brain Res Bull 75: pp. 1-14 CrossRef
  27. Petite, D, Calvet, MC (1995) Cryo-preserved neuronal cells in long-term cultures of dissociated rat cerebral cortex: survival and morphometric characteristics as revealed by immunocytochemistry. Brain Res 669: pp. 263-274 CrossRef
  28. Rahman AS, Parvinjah S, Hanna MA, Helguera PR, Busciglio J (2010) Cryopreservation of cortical tissue blocks for the generation of highly enriched neuronal cultures. J Vis Exp (45):2384
  29. Reppel, M, Pillekamp, F, Lu, ZJ, Halbach, M, Brockmeier, K, Fleischmann, BK, Hescheler, J (2004) Microelectrode arrays: a new tool to measure embryonic heart activity. J Electrocardiol 37: pp. 104-109 CrossRef
  30. Schock, SC, Jolin-Dahel, KS, Schock, PC, Theiss, S, Arbuthnott, GW, Garcia-Munoz, M, Staines, WA (2012) Development of dissociated cryo-preserved rat cortical neurons in vitro. J Neurosci Methods 205: pp. 324-333 CrossRef
  31. Silani, V, Pizzuti, A, Strada, O, Falini, A, Buscaglia, M, Scarlato, G (1988) Human neuronal cell viability demonstrated in culture after cryopreservation. Brain Res 473: pp. 169-174 CrossRef
  32. Stacey, G, MacDonald, C (2001) Immortalisation of primary cells. Cell Biol Toxicol 17: pp. 231-246 CrossRef
  33. Taupin, P (2009) Cryopreservation of early postmitotic neuronal cells in culture. Expert Opin Ther Pat 19: pp. 265-268 CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biomedicine
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-0778

文摘

Primary dissociated brain tissue from rodents is widely used in a variety of different scientific methods to investigate cellular processes in vitro. Often, for this purpose cell cultures need to be generated just on time, requiring extensive animal lab infrastructure. We show here that cryopreservation and thawing of dissociated tissue from rat cerebral cortex at embryonic day 18 is feasible without affecting its ability to form functional neuronal networks in vitro. Vitality of fresh and re-thawed cortical cells was comparable, assessed by CellTiter-Blue-assay, CytoTox-ONE assay, immunocytochemical characterization and in vitro neuronal network activity recordings on microelectrode arrays. These findings suggest that planning and execution of experiments might be considerably facilitated by using cryo-preserved neurons instead of acutely dissociated neural cultures due to fewer logistical issues with regard to animal breeding and pregnancy timed preparations.