Characterization and cross-species amplification of 19 polymorphic microsatellite loci of Hemibagrus macropterus (Bleeker) in the Yangtze River

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• 作者：Jiawen Ba (1) (2)
  Yun Li (1)
  Xiangyang Jia (2)
  Dengqiang Wang (2)
  Xinbin Duan (2)
  Daqing Chen (2)
  
  1. College of Animal Science and Technology ; Southwest University ; Chongqing ; 400716 ; China
  2. Yangtze River Fisheries Research Institute ; Chinese Academy of Fishery Science ; Wuhan ; 430223 ; China
  
• 关键词：Hemibagrus macropterus ; Microsatellite ; Genetic marker
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：7
• 期：1
• 页码：5-8
• 全文大小：161 KB
• 参考文献：1. Ding RH (1994) The fishes of Sichuan province. Sichuan Science & Technology Press, Chengdu
  2. Li Q, Wan J (2005) SSRHunter: development of a local searching software for SSR sites. Hereditas 5:808鈥?10 (in Chinese with English abstract)
  3. Ma H, He X (2004) Age and growth of / Rhinogobio cylindricus in the mainstream of Yangtze River. Chin J Zool 39:55鈥?9 (in Chinese with English abstract)
  4. World Commission on Dams (WCD) (2000) Dams and development: a new framework for decision-making. Earthscan, London
  5. Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM (2000) POPGENE 32: Microsoft Windows-based freeware for population genetic analysis. 1.32. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton
  6. Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1鈥?6 CrossRef
  
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

Hemibagrus macropterus (Bleeker) is endemic to the upper Yangtze River basin and the Zhujiang River basin in China. By using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol, we identified nineteen microsatellite loci in a wild population. The number of alleles per locus ranged from 5 to 17. The observed and expected heterozygosity ranged from 0.5132 to 1.000 and from 0.4903 to 0.9008, respectively. Cross-amplification was tested in related species Leiocassis tenuifurcatus and Pelteobagrus fulvidraco.