Resistance to gram-negative organisms due to high-level expression of plasmid-encoded ampC ¦Â-lactamase blaCMY-4 promoted by insertion sequence ISEcp1
详细信息 查看全文
- 作者：Ryuichi Nakano ; Ryoichi Okamoto ; Noriyuki Nagano and Matsuhisa Inoue
- 关键词：ISEcp1 ; Plasmid ; encoded AmpC β ; lactamase ; bla CMY ; 4 ; Citrobacter freundii
- 刊名：Journal of Infection and Chemotherapy
- 出版年：2007
- 出版时间：February, 2007
- 年：2007
- 卷：13
- 期：1
- 页码：18-23
- 全文大小：242 KB
文摘
A Klebsiella pneumoniae strain, KU6500, which showed resistance to extended-spectrum β-lactams and produced the plasmid-encoded AmpC β-lactamase CMY-4, was identified from clinical isolates in Japan. The aim of this study was to identify the mechanism of the high-level expression of bla CMY-4. Sequence analysis indicated that the promoter element of Citrobacter freundii was conserved, but the insertion sequence ISEcp1 coding with the putative promoter element, was inserted into the AmpR binding site. We determined the influence of the promoter on bla CMY-4 expression and β-lactam resistance. Two recombinant plasmids containing the entire bla CMY-4 gene, with or without the ISEcp1-mediated promoter sequences, were constructed and named pMWampC and pMWISEcp1, respectively. Escherichia coli DH5α (pMWISEcp1) was resistant to almost all β-lactams tested and E. coli DH5α (pMWampC) was susceptible to all, except for cephalothin. In addition, the activity of each promoter was measured by subcloning the element into a promoterless luciferase plasmid pGL3-Basic vector. The expression of the putative promoter of ISEcp1 was 18.9-fold higher than that of C. freundii. These results suggest that the putative promoter element of ISEcp1 is necessary for the high-level expression of bla CMY-4 to confer resistance to extended-spectrum cephalosporins.