Amelogenin promotes odontoblast-like MDPC-23 cell differentiation via activation of ERK1/2 and p38 MAPK

详细信息    查看全文

• 作者：1. Department of Stomatology ; Xinqiao Hospital ; Third Military Medical University ; Chongqing ; 400037 People’s Republic of China2. Department of Operative Dentistry and Endodontics ; School of Stomatology ; Fourth Military Medical University ; Xi’an ; Shaanxi ; 710032 People’s Republic of China3. Department of Oral & Maxillofacial Surgery ; Xinqiao Hospital ; Third Military Medical University ; Xinqiao Street ; Chongqing ; 400037 People’s Republic of China
• 关键词：Amelogenin – Odontoblast – Differentiation – ALP – DMP ; 1 – DSPP
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：355
• 期：1-2
• 页码：91-97
• 全文大小：622.0 KB
• 参考文献：1. Paine ML, Snead ML (2005) Tooth developmental biology: disruptions to enamel-matrix assembly and its impact on biomineralization. Orthod Craniofac Res 8:239–251
  2. Papagerakis P, MacDougall M, Hotton D, Bailleul-Forestier I, Oboeuf M, Berdal A (2003) Expression of amelogenin in odontoblasts. Bone 32:228–240
  3. Fincham AG, Simmer JP (1997) Amelogenin proteins of developing dental enamel. Ciba Found Symp 205:118–130
  4. Brookes SJ, Robinson C, Kirkham J, Bonass WA (1995) Biochemistry and molecular biology of amelogenin proteins of developing dental enamel. Arch Oral Biol 40:1–14
  5. Gibson CW, Yuan ZA, Hall B, Longenecker G, Chen E, Thyagarajan T, Sreenath T, Wright JT, Decker S, Piddington R, Harrison G, Kulkarni AB (2001) Amelogenin-deficient mice display an amelogenesis imperfecta phenotype. J Biol Chem 276:31871–31875
  6. Inai T, Kukita T, Ohsaki Y, Nagata K, Kukita A, Kurisu K (1991) Immunohistochemical demonstration of amelogenin penetration toward the dental pulp in the early stages of ameloblast development in rat molar tooth germ. Anat Rec 229:259–270
  7. Nanci A, McKee MD, Smith CE (1992) Immunolocalization of enamel proteins during amelogenesisin the cat. Anat Rec 233:335–349
  8. Nakamura M, Bringas P Jr, Nanci A, Zeichner-David M, Ashdown B, Slavkin HC (1996) Translocation of enamel proteins from inner enamel epithelia to odontoblast during mouse tooth development. Anat Rec 238:383–396
  9. Li Fuming Shi Junnan (1998) Immunolocalization of amelogenins expressed at different developmental stages in human tooth germ. Chin J Conserv Dent 8:43–45
  10. Veis A, Tompkins K, Alvares K, Wei K, Wang L, Wang XS, Brownell AG, Jengh SM, Healy KE (2000) Specific amelogenin gene splicing products have signaling effects on cells in culture and in implants in vivo. J Biol Chem 275:41263–41272
  11. Oida S, Nagano T, Yamakoshi Y, Ando H, Yamada M, Fukae M (2002) Amelogenin gene expression in porcine odontoblasts. J Dent Res 81:103–108
  12. Hao J, He G, Narayanan K, Zou B, Lin L, Muni T, Ramachandran A, George A (2005) Identification of differentially expressed cDNA transcripts from a rat odontoblast cell line. Bone 37:578–588
  13. Nagano T, Oida S, Ando H, Gomi K, Arai T, Fukae M (2003) Relative levels of mRNA encoding enamel proteins in enamel organ epithelia and odontoblasts. J Dent Res 82:982–986
  14. Tompkins K, Alvares K, George A, Veis A (2005) Two related low molecular mass polypeptide isoforms of amelogenin have distinct activities in mouse tooth germ differentiation in vitro. J Bone Miner Res 20:341–349
  15. Hanks CT, Sun ZL, Fang DN, Edwards CA, Wataha JC, Ritchie HH, Butler WT (1998) Cloned 3T6 cell line from CD-1 mouse fetal molar dental papillae. Connect Tissue Res 37:233–249
  16. Hao J, Narayanan K, Ramachandran A, He G, Almushayt A, Evans C, George A (2002) Odontoblast cells immortalized by telomerase produce mineralized dentin-like tissue both in vitro and in vivo. J Biol Chem 277:19976–19981
  17. Lesot H, Lisi S, Peterkova R, Peterka M, Mitolo V, Ruch JV (2001) Epigenetic signals during odontoblast differentiation. Adv Dent Res 15:8–13
  18. Thesleff I, Mikkola M (2002) The role of growth factors in tooth development. Int Rev Cytol 217:93–135
  19. Raman M, Chen W, Cobb MH (2007) Differential regulation and properties of MAPKs. Oncogene 26:3100–3112
• 作者单位：http://www.springerlink.com/content/g608x4529k1lh834/
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Medical Biochemistry
  Oncology
  Cardiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4919

文摘

Amelogenin (AMG) is a highly conserved protein secreted by ameloblasts. Some research indicates that AMG might induce the differentiation and maturation of odontoblasts. The aim of this study was to clarify the function of AMG during the differentiation of odontoblast-like MDPC-23 cells. The results revealed that the alkaline phosphatase activity and the number of mineralized nodules were significantly enhanced in AMG-overexpressing MDPC-23 cells during the mineralization process. Tissue-specific markers such as dentin matrix protein 1 and dentin sialophosphoprotein also elevated significantly, indicating the cell differentiation and maturation process. Furthermore, AMG could upregulate the phosphorylation levels of ERK1/2 and p38 MAPK. However, JNK, another MAPK pathway molecule, didn’t change the activity at all. And the differentiation induced by AMG was abrogated when the MDPC-23 cells were treated with U0126 and SB203580, the inhibitors of ERK1/2 and p38, respectively. Taken together, our present results showed that AMG could promote the differentiation of odontoblast-like MDPC-23 cells via ERK1/2 and p38 MAPK pathways.