CpG methylation in human papillomavirus (HPV) type 31 long control region (LCR) in cervical infections associated with cytological abnormalities

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• 作者：Brigitta László ; Annamária Ferenczi ; László Madar ; Eszter Gyöngyösi…
• 刊名：Virus Genes
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：52
• 期：4
• 页码：552-555
• 全文大小：705 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Virology
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-994X
• 卷排序：52

文摘

The mechanisms that regulate papillomavirus gene expression include DNA methylation. The transcription of papillomavirus oncogenes E6 and E7 is controlled by certain regulatory elements in the LCR, which include binding sites for the E2 protein, a viral regulator of oncogene expression. In HPV-31-infected exfoliated cervical cells, the CpG methylation of the entire LCR was determined by next-generation sequencing after bisulfite modification. Six of the 22 cases had methylated CpG sites in the HPV-31 LCR, including position 7479 and/or 7485, at the promoter distal E2 binding site, thus suggesting a potential regulatory mechanism for papillomavirus transcription.KeywordsHPV 31Long control region (LCR)CpG methylationE2 binding site