Production of psoralen by in vitro regenerated plants from callus cultures of Psoralea corylifolia L.

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• 作者：P. Baskaran (12) rcpgd@ukzn.ac.za
  N. Jayabalan (1)
  J. Van Staden (2)
• 关键词：Callus cultures &#8211 ; In vitro regeneration &#8211 ; Psoralea corylifolia &#8211 ; Psoralen &#8211 ; Transverse thin cell layers
• 刊名：Plant Growth Regulation
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：65
• 期：1
• 页码：47-54
• 全文大小：1.6 MB
• 参考文献：1. Baskaran P, Jayabalan N (2007) Rapid micropropagation of Psoralea corylifolia L. using nodal explants cultured in organic additive-supplemented medium. J Hortic Sci Biotechnol 82:908–913
  2. Baskaran P, Jayabalan N (2008) Effect of growth regulators on rapid micropropagation and psoralen production in Psoralea corylifolia L. Acta Physiol Plant 30:345–451
  3. Baskaran P, Jayabalan N (2009a) In vitro propagation of Psoralea corylifolia L. by somatic embryogenesis in cell suspension culture. Acta Physiol Plant 31:1119–1127
  4. Baskaran P, Jayabalan N (2009b) In vitro regeneration of Psoralea corylifolia L. through callus cultures. Plant Biotechnol 26:333–336
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• 作者单位：1. Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu 620 024, India2. Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal, Pietermaritzburg, Scottsville 3209, South Africa
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5087

文摘

Psoralea corylifolia is an attractive, endangered annual producing various bioactive compounds of medical importance. This plant contributes to Indian pharmaceutical and cosmetic industries for the production of commercial medicines, Ayurvedic skin care ointments and soap. The influence of various plant growth regulators (PGRs) and additives on high-frequency rapid adventitious shoot regeneration from transverse thin cell layer (tTCL) hypocotyl explants of P. corylifolia was investigated. Organogenic callus was obtained in tTCL hypocotyl explants on Murashige and Skoog (1962) medium supplemented with 15 μM naphthaleneacetic acid (NAA) and 3 μM benzylaminopurine (BA). The highest adventitious shoot regeneration (107.5 shoots per explant) was achieved in culture when transferred to half-strength solid MS medium. The regenerated shoots were rooted and the plantlets successfully acclimatized in moistened (1/8-MS basal salt solution with 3 μM indole-3-butyric acid (IBA), 1 μM 2-isopentenyladenine (2iP) and 100 mg l−1 Bavistin (BVN)); garden soil, farmyard soil and sand (2:1:1, v/v/v). The acclimatized plants produced flowers in the growth chamber. When planted in the field these plants set viable seed. The psoralen content in different tissues of ex vitro and naturally-grown plants was determined by high-performance liquid chromatography (HPLC). The highest psoralen content was recorded in seeds from naturally-grown (6.48 μg g−1 DW) and ex vitro plants (6.46 μg g−1 DW). This system can be used for rapid mass propagation of P. corylifolia, for conservation strategies, and to produce phytomedicines.