Physiological and morphological changes in Botrytis cinerea induced by the plant alkaloid camptothecin

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• 作者：Shengfeng Mao ; Jinyan Luo ; Zhiyi Wu ; Peng Lei ; Mengyu Ge&#8230
• 关键词：B. cinerea ; Camptothecin ; Enzyme activity ; Gene expression ; Mechanism
• 刊名：Tropical Plant Pathology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：40
• 期：4
• 页码：260-264
• 全文大小：
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• 作者单位：Shengfeng Mao (1) (4)
  Jinyan Luo (2)
  Zhiyi Wu (3)
  Peng Lei (1)
  Mengyu Ge (5)
  Bin Li (5)
  Yanli Wang (6)
  Liqin Zhang (1)
  Guochang Sun (6)
  
  1. State Key Laboratory Breeding Base for Subtropical Forest Cultivation, School of Forestry and Biotechnology, Zhejiang A & F University, 311300, Lin-an, China
  4. Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 210037, Nanjin, China
  2. Shanghai Extension and Service Center of Agriculture Technical, 201103, Shanghai, China
  3. Zhejiang Entry-Exit Inspection and Quarantine Bureau, 310012, Hangzhou, China
  5. State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, 310058, Hangzhou, China
  6. State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest and Disease Control, Key Laboratory of Detection for Pesticide Residues, Ministry of Agriculture, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, China
  
• 刊物类别：Plant Pathology;
• 刊物主题：Plant Pathology;
• 出版者：Springer International Publishing
• ISSN：1983-2052

文摘

Botrytis cinerea causes diseases in plants that lead to severe crop losses. This study aimed to evaluate the physiological and morphological changes and gene expression induced by camptothecin, a monoterpene indole alkaloid, for the control of B. cinerea. Using a single concentration of 8.0 渭g/mL, which showed to reduce 50 % of the dry weight of fungal mycelia, the alkaloid caused a 25 to 30 % reduction in colony diameter from 24 to 72 h of incubation and 34 to 54.7 % reduction in fungal biomass, from 24 to 96 h of incubation. Furthermore, changes in mycelial morphology were noticed based on both scanning and transmission electron microscopic observations. POD activity was reduced by 49.1 %, but PAL and SOD activity were not affected. Moreover, q-PCR analysis showed that the expression of erg27, os1, and SDHB were not affected, but the expression of cytb increased significantly, thus suggesting the involvement of this respiration-related gene in the response of B. cinerea to camptothecin. In summary, the alkaloid has the potential to improve the control of gray mold caused by B. cinerea. Keywords B. cinerea Camptothecin Enzyme activity Gene expression Mechanism