文摘
Botrytis cinerea causes diseases in plants that lead to severe crop losses. This study aimed to evaluate the physiological and morphological changes and gene expression induced by camptothecin, a monoterpene indole alkaloid, for the control of B. cinerea. Using a single concentration of 8.0 渭g/mL, which showed to reduce 50 % of the dry weight of fungal mycelia, the alkaloid caused a 25 to 30 % reduction in colony diameter from 24 to 72 h of incubation and 34 to 54.7 % reduction in fungal biomass, from 24 to 96 h of incubation. Furthermore, changes in mycelial morphology were noticed based on both scanning and transmission electron microscopic observations. POD activity was reduced by 49.1 %, but PAL and SOD activity were not affected. Moreover, q-PCR analysis showed that the expression of erg27, os1, and SDHB were not affected, but the expression of cytb increased significantly, thus suggesting the involvement of this respiration-related gene in the response of B. cinerea to camptothecin. In summary, the alkaloid has the potential to improve the control of gray mold caused by B. cinerea. Keywords B. cinerea Camptothecin Enzyme activity Gene expression Mechanism