Evidence that ghrelin may be associated with the food intake of gibel carp (Carassius auratus gibelio)

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• 作者：Chaowei Zhou ; Jinfa Zheng ; Luo Lei ; Dengyue Yuan&#8230
• 关键词：Gibel carp ; Ghrelin ; Tissue expression ; Appetite regulation
• 刊名：Fish Physiology and Biochemistry
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：42
• 期：6
• 页码：1637-1646
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Freshwater & Marine Ecology; Animal Physiology; Animal Anatomy / Morphology / Histology; Animal Biochemistry; Zoology;
• 出版者：Springer Netherlands
• ISSN：1573-5168
• 卷排序：42

文摘

Ghrelin, a non-amidated peptide hormone, is a potent anorectic neuropeptide implicated in feeding regulation in mammals and non-mammalian vertebrates. However, the involvement of ghrelin in the feeding behavior of teleosts has not been well understood. To better understand the role of ghrelin in the regulation of appetite in fish, in this study, we cloned the cDNAs encoding ghrelin and investigated their mRNA distributions in gibel carp tissues. We also assessed the effects of different nutritional status on ghrelin mRNA abundance. Ghrelin mRNAs were ubiquitously expressed in ten tissues (intestine, liver, brain, mesonephron, head kidney, spleen, skin, heart, muscle, gill and pituitary gland), and relatively high expression levels were detected in the gut. Postprandial studies analysis revealed a significant postprandial decrease in ghrelin mRNA expression in the gut (1 and 3 h after the regular feeding time). In addition, ghrelin mRNA expression in the gut significantly increased at day 7 after fasting and declined sharply after refeeding, which suggested that ghrelin might be involved in the regulation of appetite in gibel carp. Overall, our result provides basis for further investigation into the regulation of feeding in gibel carp.