A sensitive immunoassay for parathion based on covalent linkage between small molecules hapten microtiter plates surface
详细信息 查看全文
- 作者：Na Sai ; Wenjing Sun ; Yuntang Wu ; Zhong Sun…
- 关键词：Competive inhibition enzyme ; linked immunosorbent assay ; Covalent linkage ; Parathion ; Small molecule hapten
- 刊名：Journal of the Iranian Chemical Society
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：14
- 期：1
- 页码：257-268
- 全文大小：
- 刊物主题：Analytical Chemistry; Inorganic Chemistry; Physical Chemistry; Biochemistry, general; Organic Chemistry;
- 出版者：Springer Berlin Heidelberg
- ISSN：1735-2428
- 卷排序：14
文摘
A sensitive competitive inhibition enzyme-linked immunosorbent assay (CIELISA) for the detection of parathion has been developed. In this assay, a small molecule hapten II (O,O-diethyl O-4-aminophenyl phosphorothioate) was covalent linked to glutaric dialdehyde treated-microtiter plates. In addition, 4-(ethoxy(4-nitrophenoxy) phosphorothioylamino) butanoic acid-ovalbumin (hapten I–OVA) conjugate served as the coating antigen for comparison with directly hapten II covalent linked plates in the CIELISA format. The developed assay demonstrated highly sensitivity (IC10 was 0.08 ng mL−1) selectivity and stability. In samples analysis, the results of parathion detected by this assay were in accordance with which obtained by high-performance liquid chromatography.