Proteomic Profiling of the Aleurone Layer of Mature Arabidopsis thaliana Seed

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• 作者：Abdur Rashid (1) (3)
  Ajay Badhan (1)
  Michael Deyholos (1)
  Nat Kav (2)
  
• 关键词：Arabidopsis thaliana ; Seed coat ; Aleurone layer ; Proteomic profile ; 2D ; gel electrophoresis ; LC ; MS/MS ; AGI loci ; Seed storage proteins
• 刊名：Plant Molecular Biology Reporter
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：31
• 期：2
• 页码：464-469
• 全文大小：307KB
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• 作者单位：Abdur Rashid (1) (3)
  Ajay Badhan (1)
  Michael Deyholos (1)
  Nat Kav (2)
  
  1. Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada, T6G 2E9
  3. Crop Diversification Centre (North), Alberta Agriculture and Rural Development, 17507 Fort Road, Edmonton, AB, Canada, T5Y 6H3
  2. Department of Agriculture, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada, T6G 2H1
  
• ISSN：1572-9818

文摘

The aleurone layer of mature Arabidopsis thaliana seed plays important roles in seed germination and dormancy. However, the proteomic profile of this cell layer is unknown partly because it is difficult to separate this thin cell layer from the mature seeds. In this study, we have used a simple technique to separate the aleurone layer along with the seed coat following germination of seeds and determined for the first time the putative protein composition of this cell layer. By subjecting the total proteins extracted from the seed coat to 2D gel electrophoresis followed by liquid chromatography/tandem mass spectrometry, we identified four AGI loci, AT4G28520, AT5G44120, AT1G03880, and AT1G03890; all of which belong to the seed storage family of proteins. Because in Arabidopsis the diploid aleurone cells of the seed coat perform protein storage functions similar to that of triploid endosperm of other plant species, it is assumed that the above AGI loci are associated with the aleurone layer of the seed coat.